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Analysis of methicillin-resistant Staphylococcus aureus by IS1181 profiling

Published online by Cambridge University Press:  01 June 1998

C. SYMMS ,
B. COOKSON ,
J. STANLEY  and
J. V. HOOKEY
C. SYMMS
Affiliation:
Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London, NW9 5HT
B. COOKSON
Affiliation:
Laboratory of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London, NW9 5HT
J. STANLEY
Affiliation:
Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London, NW9 5HT
J. V. HOOKEY
Affiliation:
Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London, NW9 5HT
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Abstract

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Variation in the genomic location and copy number of the insertion element IS1181 in methicillin-resistant Staphylococcus aureus (MRSA) was investigated. Sixty-three isolates representing the Jevons type strain (NCTC 10442), phage-propagating strains, and epidemic strains were examined. A PCR amplicon of the insertion element was used to probe genomic restriction endonuclease digests. HindIII genomic digests gave 25 distinct IS1181 patterns, while EcoRI digests gave 20 patterns. EMRSA-01, -02, -04, -06, -07, -09, -10, -11, -13 and -14 contained the element but could not be subtyped by profiling it. EMRSA-16 did not contain IS1181, consistent with a unique evolutionary origin for this major UK epidemic strain. Marked heterogeneity was observed among isolates of EMRSA-03. Each EMRSA-03 strain examined gave a unique pattern, thereby allowing subtyping of an important epidemic phage type for the purposes of hospital cross-infection control.

Type
Research Article
Information
Epidemiology & Infection , Volume 120 , Issue 3 , June 1998 , pp. 271 - 279
Copyright
1998 Cambridge University Press