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An investigation of colonization of the conjunctival sac of sheep by bacteria and mycoplasmas

Published online by Cambridge University Press:  15 May 2009

G. J. R. Dagnall
G. J. R. Dagnall
Affiliation:
The Royal Veterinary College, Department of Animal Health, Boltons Park, Potters Bar, Hertfordshire EN6 1NB
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Summary

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The conjunctival sacs of 100 healthy adult ewes and 20 lambs and 76 adult ewes affected by ovine keratoconjunctivitis (OKC) were examined microbiologically. Branhamella ovis was the only organism regularly isolated from healthy ewes and lambs. Mycoplasma conjunctivae was isolated from the conjunctival sac of nine ewes but its occurrence was not associated with clinical signs of ovine keratoconjunctivitis. Mycoplasma conjunctivae and Branhamella ovis were isolated significantly more often from eyes affected by OKC than from unaffected eyes.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 3 , June 1994 , pp. 561 - 567
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

1.Spradbrow, PB. The bacterial flora of the ovine conjunctival sac. Aust Vet J 1968; 44: 117–18.CrossRefGoogle ScholarPubMed
2.Egwu, GO, Faull, WB, Bradbury, JM, Clarkson, MJ. Ovine infectious keratoconjunctivitis: a microbiological study of clinically unaffected and affected sheep's eyes with special reference to Mycoplasma conjunctive. Vet Rec 1989; 125: 253–6.CrossRefGoogle Scholar
3.Barile, MF, del Giudice, RA, Tully, JG. Isolation and characteristics of Mycoplasma conjunctivae sp. n. from sheep and goats with keratoconjunctivitis. Infect Immun 1972; 5: 70–6.CrossRefGoogle Scholar
4.Jones, GE, Foggie, A, Sutherland, A, Marker, DB. Mycoplasmas and ovine keratoconjunctivitis. Vet Rec 1976; 99: 137–41.CrossRefGoogle ScholarPubMed
5.Laak, EA ter, Schreuder, BEC, Smith-Buys, CMC. The occurrence of Mycoplasma conjunctivae in the Netherlands and its association with infectious keratoconjunctivitis in sheep and goats. Vet Q 1988; 10: 73–83.CrossRefGoogle ScholarPubMed
6.Dagnall, GJR. A study of ovine keratoconjunctivitis with special reference to Mycoplasma conjunctivae and Branhamdla ovis. [PhD thesis]. Royal Veterinary College. London: University of London. 1991.Google Scholar
7.Woodland, RM, Wilsmore, AJ, Dagnall, GJR. Sheep conjunctivitis caused by Chlamydia psittaci and Branhamella ovis. Proc Eur Soc Chlamydia Res 1988; 1: 134.Google Scholar
8.Cello, RM. Ocular infection in animals with PLT (Bedsonia) group agents. Am J Ophthal 1967; 63: 1270–4.CrossRefGoogle ScholarPubMed
9.Storz, J, Pierson, RE, Marriott, ME, Chow, TL. Isolation of psittacosis agents from follicular conjunctivitis in sheep. Proc Soc Exp Biol Med 1967; 125: 857–60.CrossRefGoogle Scholar
10.Andrews, AH, Goddard, PC, Wilsmore, AJ, Dagnall, GJR. A chlamydial keratoconjunctivitis in a British sheep flock. Vet Rec 1987; 120: 238–9.CrossRefGoogle Scholar
11.Woodland, RM, Kirton, RP, Darougar, S. Sensitivity of mitomycin-C treated McCoy cells for isolation of Chlamydia trachomatis from genital tract specimens. Eur J Clin Microbiol 1987; 6: 653–6.CrossRefGoogle Scholar
12.Sims, W. Oral haemophili. J Med Microbiol 1970; 3: 615–25.CrossRefGoogle ScholarPubMed
13.Cowan, ST, ed. Cowan and Steel's manual for the identification of medical bacteria. 2nd ed., Cambridge: Cambridge University Press, 1974.Google Scholar
14.Freundt, EA. Culture media for classic mycoplasmas. In: Razin, S, Tully, JG, eds. Methods in mycoplasmology. vol 1. Mycoplasma characterization. New York: Academic Press, 1983: 130–1.Google Scholar
15.Clyde, WA. Mycoplasma species identification based upon growth inhibition by specific antisera. J Immun 1964; 92: 958–65.CrossRefGoogle ScholarPubMed
16.Rosendal, S, Black, FT. Direct and indirect immunofluorescence of unfixed and fixed mycoplasma colonies. Acta Path Microbiol Scand 1972; 80B: 615–22.Google Scholar
17.Lindqvist, K. A neisseria species associated with infectious keratoconjunctivitis of sheep Neisseria ovis Nov. Spec. J Infect Dis 1960; 106: 162–5.CrossRefGoogle ScholarPubMed
18.Dagnall, GJR. Some cultural characteristics of Branhamella ovis isolated from the conjunctival sac of sheep. Vet Microbiol 1993; 37: 45–52.CrossRefGoogle ScholarPubMed
19.Nicolet, J von, Dauwalder, M, Boss, PH, Anetzhofer, J. Die “primar” infektiöse Keratoconjunctivitis des Rindes. Mögliche ätiologishe Rolle von Mycoplasma bovoculi. Schweizer Arch Tierheilk 1976; 118: 141–50.Google ScholarPubMed