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An improved assay method for neutralizing antibodies against influenza viruses

Published online by Cambridge University Press:  15 May 2009

S. Fazekas de St Groth ,
J. Withell  and
K. J. Lafferty
S. Fazekas de St Groth
Affiliation:
From the Department of Microbiology, Australian National University, Canberra
J. Withell
Affiliation:
From the Department of Microbiology, Australian National University, Canberra
K. J. Lafferty
Affiliation:
From the Department of Microbiology, Australian National University, Canberra
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1. A method has been developed for the assay of antisera against influenza viruses.

2. Surviving bits of allantois-on-shell serve as host tissue, eighty to one hundred units being provided by a single egg. The test is set up in plastic trays, and uses a simple synthetic medium.

3. The method has worked equally well with the ten representative strains of influenza virus tested. It surpasses the orthodox techniques of neutralization in mice or whole eggs as regards sensitivity, accuracy and economy.

Type
Research Article
Information
Journal of Hygiene , Volume 56 , Issue 3 , September 1958 , pp. 415 - 426
Copyright
Copyright © Cambridge University Press 1958

References

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