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An evaluation of commercially available dehydrated Rappaport-Vassiliadis medium for the isolation of salmonellae from poultry

Published online by Cambridge University Press:  19 October 2009

C. R. Fricker ,
Elaine Quail ,
Loraine McGibbon  and
R. W. A. Girdwood
C. R. Fricker
Affiliation:
Department of Bacteriology, Stobhill General Hospital, Glasgow G21 3UW
Elaine Quail
Affiliation:
Department of Bacteriology, Stobhill General Hospital, Glasgow G21 3UW
Loraine McGibbon
Affiliation:
Department of Bacteriology, Stobhill General Hospital, Glasgow G21 3UW
R. W. A. Girdwood
Affiliation:
Department of Bacteriology, Stobhill General Hospital, Glasgow G21 3UW
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A total of 745 samples of chicken giblets was cultured to determine the relative efficiency of a commercially available Rappaport-Vassiliadis medium (RV-Oxoid). Experiments to determine the optimum inoculation ratio showed that 1:100 was superior to the other ratios tested. Comparison of RV-Oxoid with standard RV and RV-medium prepared using soya peptone (RV-soya) showed that after 24 h RV-soya was significantly better than RV-Oxoid (P < 0·05), although there was no significant difference between standard RV and RV-Oxoid. Furthermore, when the duration of incubation was extended to 48 h there was no significant difference between the three media (P > 0·25).

We conclude that RV-Oxoid is a satisfactory product for the isolation of salmoncllae from poultry, providing that it is inoculated at a ratio of 1:100 and is incubated for 48h. Its use can therefore be recommended to laboratories who wish to use a dehydrated medium.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 2 , October 1985 , pp. 337 - 344
Copyright
Copyright © Cambridge University Press 1985

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