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An epidemic methicillin-resistant strain of Staphylococcus aureus in Spain

Published online by Cambridge University Press:  15 May 2009

P. Aparicio ,
J. Richardson ,
S. Martin ,
A. Vindel ,
R. R. Marples  and
B. D. Cookson
P. Aparicio
Affiliation:
Laboratorio de Referencia de Estafilococos, Centro National de Microbiologia, Majadahonda, Madrid, Spain Division of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London, UK
J. Richardson*
Affiliation:
Division of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London, UK
S. Martin
Affiliation:
Division of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London, UK
A. Vindel
Affiliation:
Laboratorio de Referencia de Estafilococos, Centro National de Microbiologia, Majadahonda, Madrid, Spain
R. R. Marples
Affiliation:
Division of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London, UK
B. D. Cookson
Affiliation:
Division of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London, UK
*
*Dr J. F. Richardson, Staphylococcus Reference Laboratory. Central Public Health Laboratory. 61 Colindale Avenue. London NW9 5HT.
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Summary

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During 1990, a strain of methicillin-resistant Staphylococcus aureus became epidemic in Spain and spread in a manner analogous to that of EMRSA-1 in England. Isolates of this strain produced little protein A and were resistant to a number of antibiotics including ciprofloxacin. Beta-lactamase production was encoded by a c. 39 kb plasmid, which also conferred resistance to mercury, cadmium, ethidium bromide and propamidine isethionate.

Investigation showed that two variants, separable by supplementary and Fisk phage typing, were circulating. The B variant appeared to spread more readily than the A variant.

The opportunity was taken to compare the discriminatory power of traditional typing methods with molecular techniques. The discriminatory power of the molecular techniques used only reached the same level as the traditional methods when double enzyme digestion of total cellular DNA by EcoR I and Cla I was performed.

Type
Research Article
Information
Epidemiology & Infection , Volume 108 , Issue 2 , April 1992 , pp. 287 - 298
Copyright
Copyright © Cambridge University Press 1992

References

REFERENCES

1.Williams, REO. Epidemic staphylococci. Lancet 1959; i: 190–5.CrossRefGoogle Scholar
2.Townsend, DE, Ashdown, N, Bolton, S et al. , The international spread of methicillin-resistant Staphylococcus aureus. J Hosp Infect 1987; 9: 60–71.CrossRefGoogle ScholarPubMed
3.Marples, RR, Cooke, EM. Current problems with methicillin-resistant Staphylococcus aureus. J Hosp Infect 1988; 11: 381–92.CrossRefGoogle ScholarPubMed
4.Kerr, S, Kerr, GE, Mackintosh, CA, Marples, RR. A survey of methicillin-resistant Staphylococcus aureus in England and Wales. J Hosp Infect 1990; 16: 35–48.Google Scholar
5.Marples, RR. Methicillin-resistant Staphylococcus aureus Curr Opin Inf Dis 1988; 1: 722–6.CrossRefGoogle Scholar
6.Bouza, E, Martinez-Beltran, J. Estudio multicentro sobre la prevalencia de estafilocoeos en Espana (informe preliminar). Enfermedardes Infecciosas y Microbiologia Clinica 1988; 6: 68–79.Google Scholar
7.Phillips, I, Andrews, JM, Burt, AJ et al. , A guide to sensitivity testing. J Antimicrob Chemother 1991; 27 Suppl D: 29.Google Scholar
8.Burnet, FM. The production of staphylococcal toxin. J Pathol Bacteriol 1930; 33: 1–16.CrossRefGoogle Scholar
9.Novick, R. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology 1967; 33: 155–166.CrossRefGoogle ScholarPubMed
10.Jevons, MP.‘Celbenin’-resistant staphylococci. B M J 1961; 1: 124–5.Google Scholar
11.Kloos, WE, Schleifer, KH. Isolation and characterization of staphylococci from the human skin. II Description of four new species: Staphylococcus warneri. Staphylococcus capitis. Staphylococcus hominis and Staphylococcus simulans. Int J Syst Bacteriol 1975; 25: 62–79.CrossRefGoogle Scholar
12.Marples, RR, Richardson, JF, de Saxe, MJ. Bacteriological characters of strains of Staphylococcus aureus submitted to a reference laboratory related to methicillin resistance. J Hyg 1986; 96: 217–23.CrossRefGoogle ScholarPubMed
13.Richardson, JF, Marples, RR. Another strain of methicillin-resistant Staphylococcus aureus epidemic in London. Lancet 1988; ii: 748–9.Google Scholar
14.Winkler, KCde Waarf, J, Grootsen, C, Zegers, BJM, Tellier, NF, Wertregt, CD. Lysogenic conversion of staphylococci to loss of β toxin. J Gen Microbiol 1965; 39: 321–33.Google Scholar
15.Blair, JE, Williams, REO. Phage typing of staphylococci. Bull World Hlth Org 1961; 24: 771–84.Google ScholarPubMed
16.Subcommittee on the phage typing of staphylococci. Int J Syst Bact 1975; 25: 67–74.Google Scholar
17.Richardson, JF, Chittasobhon, N, Marples, RR. A supplementary phage set for the investigation of methicillin-resistant strains of Staphylococcus aureus J Med Microbiol 1988; 25: 67–74.CrossRefGoogle Scholar
18.Fisk, RT. Studies on staphylococci II. Identification of Staphylococcus aureus strains by means of bacteriophage. J Infect Dis 1942; 71: 161–5.CrossRefGoogle Scholar
19.De Saxe, MJ, Notley, CM. Experiences with the typing of coagulase-negative staphylococci and micrococci. Zbl Bakt Hyg I Abt. Orig A 1978; 241: 46–59.Google ScholarPubMed
20.Vickery, AM, Beard-Pegler, MA, Rountree, PM. Strain differentiation in methicillin-resistant Staphylococcus aureus. Pathology 1983; 15: 235–40.CrossRefGoogle ScholarPubMed
21.Rippon, JE. The classification of bacteriophages lysing staphylococci. J Hyg 1956; 54. 213–26.CrossRefGoogle ScholarPubMed
22.Simkovicova, M, Gilbert, RJ. Serological detection of enterotoxin from food poisoning strains of Staphylococcus aureus. J Med Microbiol 1971; 4: 19–30.Google Scholar
23.Cowan, ST. Cowan and Steel's manual for the identification of medical bacteria. 2nd edn.Cambridge: Cambridge University Press, 1974.Google Scholar
24.Richardson, JF, Quoraishi, AHM, Francis, BJ, Marples, RR.Beta-lactamase negative methicillin-resistant Staphylococcus aureus in a newborn nursery: report of an outbreak and laboratory investigations. J Hosp Infect 1990; 16: 109–21.CrossRefGoogle Scholar
25.Ericsson, H. Standardisation of methods for conducting sensitivity tests. Stockholm, Sweden: Karolinska Sjkhuset, 1964.Google Scholar
26.Richardson, JF, Marples, RR. Differences in antibiotic susceptibility between Staphylococcus epidermidis and Staphylococcus saprophyticus. J Antimicrob Chemother 1980; 6: 499–510.Google Scholar
27.Richardson, JF, Marples, RR, Kerr, GE. Epidemiological typing of Staphylococcus aureus. Zbk Bakt Suppl 1991; 21: 389–93.Google Scholar
28.Maniatis, T, Fitsch, EF, Sambrook, J.Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Press, 1982.Google Scholar
29.Pitcher, DG, Owen, RJ, Dyal, P, Beck, A. Synthesis of a biotinylated DNA probe to detect ribosomal DNA cistrons in Providencia stuartii. FEMS Microbiol Lett 1987; 48: 283–7.Google Scholar
30.Richardson, JF, Noble, WC, Marples, RR. Identification and epidemiological typing of the staphylococci. In: Identification methods in applied and environmental microbiology. Skinner, FA, Jones, D, eds. Society for Applied Bacteriology. Technical Series 29. In press.Google Scholar