Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-28T21:32:05.209Z Has data issue: false hasContentIssue false
  • English
  • Français

An alternative method of phage-typing Staphylococcus aureus*

Published online by Cambridge University Press:  15 May 2009

J. V. S. Pether
J. V. S. Pether
Affiliation:
Cross-Infection Reference Laboratory, Central Public Health Laboratory, London, N. W. 9
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

As many cultures of Staph. aureus untypable by the international basic-set phages at RTD were typed by a block of 20 extra phages at RTD as by the basic set at RTD × 1000. Reproducibility of the lytic patterns was as good with the extra phages at RTD as with the basic-set phages at RTD, and better than with the basic-set phages at RTD × 1000.

A method of assessing redundancy in any set of phages is presented, and a ‘3-strong differences’ rule is suggested for discriminating between cultures of staphylococci from patterns of phage lysis by the combined set of phages at RTD.

Experience with the combined set of phages suggests that it may be possible to improve the phage-typing of staphylococci by substituting a larger series of phages used only at RTD, for the present set of 22 phages used at RTD and RTD × 1000.

Type
Research Article
Information
Journal of Hygiene , Volume 66 , Issue 4 , December 1968 , pp. 605 - 623
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Beard, M. A. & Rountree, P. M. (1965). Bacteriophage inhibition in staphylococci. J. gen. Microbiol. 40, 207.Google Scholar
Blair, J. E. & Williams, R. E. O. (1961). Phage typing of staphylococci. Bull. Wld Hlth Org. 24, 771.Google ScholarPubMed
Burnet, F. M. & Lush, D. (1935). The staphylococcal bacteriophages. J. Path. Bact. 40, 455.CrossRefGoogle Scholar
Comtois, R. D. (1960). Changes in the phage-typing patterns of staphylococci following lysogenisation with a related group of staphylococcus bacteriophages. Can. J. Microbiol. 6, 491.CrossRefGoogle Scholar
Davidson, I. (1961). A set of bacteriophages for typing bovine staphylococci. Res. vet. Sci. 2, 396.CrossRefGoogle Scholar
Davidson, I. (1966). The phage-typing of bovine staphyloccoci. V. Colloquium über Fragen der Lysotypie, Wernigerode, p. 155.Google Scholar
Degré, M. (1966). Bacteriophage typing of Staphylococcus aureus using a single concentration of phage (100 × RTD). Acta path. microbiol. scand. 66, 105.Google Scholar
Degré, M. (1967). Reproducibility of staphylococcal bacteriophage types by use of different concentrations of bacteriophage. Appl. Microbiol. 15, 907.CrossRefGoogle ScholarPubMed
Hershey, A. D., Kalmanson, G. & Bronfenbrenner, J. (1943). Quantitative methods in the study of the phage-antiphage reaction. J. Immun. 46, 267.Google Scholar
Lidwell, O. M. (1959). Apparatus for phage-typing of Staphylococcus aureus. Mon. Bull. Minist. Hlth 18, 49.Google Scholar
Lowbury, E. J. L. & Hood, A. M. (1953). Acquired resistance of Staphylococcus aureus to bacteriophage. J. gen. Microbiol. 9, 524.CrossRefGoogle ScholarPubMed
Meyer, W. (1967). Staphylococcus aureus strains of phage-group IV. J. Hyg., Camb. 65, 439.Google Scholar
Parker, M. T. (1966). The uses of Staphylococcus phage typing. V. Colloquium über Fragen der Lysotypie, Wernigerode, p. 144.Google Scholar
Pöhn, H.-Ph. (1957). Die Spezifität der Typenphagen für die einzelnen Staphylokokken-Gruppen. Zentbl. Bakt. ParasitKde. (I Abt. Orig.) 168, 533.Google Scholar
Ralston, D. J., Baer, B. S., Lieberman, M. & Krueger, A. P. (1957). Lysis from without of Staphylococcus aureus K1 by the combined action of phage and virolysin. J. gen. Physiol. 41, 343.Google Scholar
Report (1967). Minutes of the 4th meeting of the International Subcommittee on Staphylococcus Phage-Typing, Moscow 1966. Int. J. system. Bact. 17, 113.Google Scholar
Rosenblum, E. D. & Dowell, C. E. (1960). Lysogeny and bacteriophage typing in coagulase positive staphylococci. J. infect. Dis. 106, 297.CrossRefGoogle ScholarPubMed
Rountree, P. M. (1947). Staphylococcal bacteriophages. II. Bacteriophage absorption by staphylococci. Aust. J. exp. Biol. med. Sci. 25, 203.CrossRefGoogle Scholar
Rountree, P. M. (1949). The phenomenon of lysogenicity in staphylococci. J. gen. Microbiol. 3, 153.CrossRefGoogle ScholarPubMed
Swanstrom, M. & Adams, M. H. (1951). Agar layer method for production of high titre phage stocks. Proc. Soc. exp. Biol. Med. 78, 372.Google Scholar
Thomas, D. C., Hill, E. O., Culbertson, W. R. & Altemeier, W. A. (1960). Development of new bacteriophages for staphylococcal typing. Surg. Forum 10, 334.Google Scholar
Wallmark, G. (1954). Bacteriophage typing of Staphylococcus aureus pyogenes. Acta path. microbiol. scand. 34, 57.Google Scholar
Wallmark, G. & Finland, M. (1961). New phages active against staphylococci that are resistant to the conventional phage set. Proc. Soc. exp. Biol. Med. 106, 73.Google Scholar
Williams, R. E. O. & Rippon, J. E. (1952). Bacteriophage typing of Staphylococcus aureus. J. Hyg., Camb. 50, 320.CrossRefGoogle ScholarPubMed
Williams, R. E. O., Rippon, J. E. & Dowsett, L. M. (1953). Bacteriophage typing of strains of Staphylococcus aureus from various sources. Lancet i, 510.Google Scholar
Wilson, G. S. & Atkinson, J. D. (1945). Typing of staphylococci by the bacteriophage method. Lancet i, 647.CrossRefGoogle Scholar
Zierdt, C. H. & Marsh, H. H. (1962). Optimal concentration of phage for typing of staphylococci. Am. J. clin. Path. 38, 104.Google Scholar