Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-22T15:45:06.208Z Has data issue: false hasContentIssue false
  • English
  • Français

An abattoir-based study of the prevalence of subclinical Johne's disease in adult cattle in south west England

Published online by Cambridge University Press:  15 May 2009

B. Çetinkaya ,
K. Egan ,
D. A. Harbour  and
K. L. Morgan
B. Çetinkaya
Affiliation:
Epidemiology Group, Division of Animal Health and Husbandry, University of Bristol, Landford House, Landford, Bristol BS18 7DU
K. Egan
Affiliation:
Epidemiology Group, Division of Animal Health and Husbandry, University of Bristol, Landford House, Landford, Bristol BS18 7DU
D. A. Harbour
Affiliation:
Epidemiology Group, Division of Animal Health and Husbandry, University of Bristol, Landford House, Landford, Bristol BS18 7DU
K. L. Morgan*
Affiliation:
Epidemiology Group, Division of Animal Health and Husbandry, University of Bristol, Landford House, Landford, Bristol BS18 7DU
*
* Author for correspondence. Current address: Department of Veterinary Clinical Science and Animal Husbandry, University of Liverpool, Leahurst, South Wirral L64 7TE
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The prevalance of subclinical Johne's disease was estimated in adult cattle slaughtered at three major abattoirs in south west England. A polymerase chain reaction (PCR) based on IS900 was used to detect Mycobacterium paratuberculosis in intestinal lymph nodes of 1553 cattle. Culture was also carried out on all PCR positive and inconclusive samples. The prevalence of subclinical disease in adult cattle was 3·5% (95% confidence intervals (CI) 2·6–4·7) by PCR and 2·6% (CI 1·8–3·6) by culture. The proportion of the disease in each month ranged from 1·6% (CI 0·2–5·5) in April to 4·6% (CI 2·8–6·9) in November, but the difference was not significant (P > 0·05). The proportion of PCR positive lymph nodes in each abattoir ranged from 2·8% (CI 1·6–4·6) to 4·9% (CI 2·9–7·6), this difference was not significant either (P > 0·05). The prevalence in young cattle was 2·0% (CI 0·6–4·5). The difference between age groups was not statistically significant (P > 0·05).

Type
Special Article
Information
Epidemiology & Infection , Volume 116 , Issue 3 , June 1996 , pp. 373 - 379
Copyright
Copyright © Cambridge University Press 1996

References

REFERENCES

1.Johne, HA, Frothingham, L. Ein eigenthuemlicher fall von tuberkulose beim rind. (A peculiar case of tuberculosis in a cow). Dt A Tiermed Verg Path 1895; 21: 438–54.Google Scholar
2.Smith, HW. The isolation of mycobacteria from the mesenteric lymph nodes of domestic animals. J Path Bact 1954; 68: 367–72.CrossRefGoogle ScholarPubMed
3.Riemann, HP, Abbas, B. Diagnosis and control of bovine paratuberculosis (Johne's disease). Adv Vet Sci Comp Med 1983; 27: 481–506.Google ScholarPubMed
4.Chiodini, RJ, Van Kruiningen, HJ, Merkal, RS. Ruminant paratuberculosis (Johne's disease): The current status and future prospects. Cornell Vet 1984; 74: 218–62.Google ScholarPubMed
5.Benedictus, G, Dijkhuizen, AA, Stelwagen, J. Economic losses due to paratuberculosis in dairy cattle. Vet Rec 1987; 121: 142–6.CrossRefGoogle ScholarPubMed
6.Thoen, CO, Baum, KH. Current knowledge on paratuberculosis. JAVMA 1988; 192: 1609–11.Google ScholarPubMed
7.Morgan, KL. Johne's and Crohn's: Chronic inflammatory bowel diseases of infectious aetiology? Lancet 1987; i: 1017–19.CrossRefGoogle Scholar
8.Chiodini, RJ, Van Kruiningen, HJ, Merkal, RS, Thayer, WR, Coutu, JA. Characteristics of an unclassified Mycobacterium species isolated from patients with Crohn's disease. J Clin Microbiol 1984; 24: 966–71.CrossRefGoogle Scholar
9.Hermon-Taylor, J, Moss, M, Tizard, M, Malik, Z, Sanderson, J. Molecular biology of Crohn's disease mycobacteria. Bailliere's Clin Gastroenterol 1990; 4: 23–12.CrossRefGoogle ScholarPubMed
10.Green, EP, Tizard, MLV, Moss, MT et al. , Sequence and characteristics of IS900, an insertion element identified in a human Crohn's disease isolate of Mycobacterium paratuberculosis. Nucleic Acids Res 1989; 17: 9063–72.CrossRefGoogle Scholar
11.Vary, PH, Anderson, PR, Green, E, Hermon-Taylor, J, McFadden, JJ. Use of a highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease. J Clin Microbiol 1990; 28: 933–37.CrossRefGoogle ScholarPubMed
12.Moss, MT, Sanderson, JD, Tizard, MLV et al. , Polymerase chain reaction detection of Mycobacterium paratuberculosis and Mycobacterium avium subsp silvaticum in long term cultures from Crohn's disease and control tissues. Gut 1992; 33: 1209–13.CrossRefGoogle ScholarPubMed
13.Sanderson, JD, Moss, MT, Tizard, MV, Hermon-Taylor, J. Mycobacterium paratuberculosis DNA in Crohn's disease tissue. Gut 1992; 33: 890–6.CrossRefGoogle ScholarPubMed
14.Çetinkaya, B, Egan, K, Morgan, KL. A practice-based survey of the frequency of Johne's disease in south west England. Vet Rec 1994; 134: 494–7.CrossRefGoogle ScholarPubMed
15.Vandegraff, R, Barton, MD, Barry, GR, van Wijk, JGA. Prevalence of Mycobacterium paratuberculosis in dairy cattle in South Australia. In: Chiodini, RJ, Collins, MT, Bassey, EOE, eds. Proc of the Fourth Int Coll on Paratuberculosis, Cambridge, 1994; 9–18.Google Scholar
16.Jorgensen, JB. On the occurrence of Mycobacterium johnei in the mesenteric lymph nodes of abattoir cattle. Nord Vet Med 1965; 17: 97–102.Google Scholar
17.Merkal, RS, Whipple, DL, Sacks, JM, Synder, GR. Prevalence of Mycobacterium paratuberculosis in ileocecal lymph nodes of cattle culled in the United States. JAVMA 1987; 190: 676–80.Google ScholarPubMed
18.Chiodini, RJ, Van Kruiningen, HJ. The prevalence of paratuberculosis in culled New England cattle. Cornell Vet 1986; 76: 91–104.Google ScholarPubMed
19.Taylor, AW. Observations on the incidence of infection with M. johnei in cattle. Vet Rec 1949; 61: 539–40.Google Scholar
20.Cannon, RM, Roe, RT. Livestock disease surveys: A field manual for veterinarians. Canberra, Australian Bureau of Animal Health, 1982.Google Scholar
21.Withers, FW. II. Incidence of the disease. Vet Rec 1959; 71: 1150–3.Google Scholar
22.Meat and Livestock Commission (MLC). Licensed red meat slaughterhouses and cattle slaughterings in the UK, 1994.Google Scholar
23.Challans, JA, Stevenson, K, Reid, HW, Sharp, JM. A rapid method for the extraction and detection of Mycobacterium avium subspecies paratuberculosis from clinical specimens. Vet Rec 1994; 134: 95–6.CrossRefGoogle ScholarPubMed
24.Herring, AJ, Inglis, NF, Ojeh, CK, Snodgrass, DR, Menzies, JD. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrilamide gels. J Clin Microbiol 1982; 16: 473–7.CrossRefGoogle Scholar
25.Dean, AG, Dean, AJ, Coulombier, D et al. , Epi-Info, Version 6: a word processing, database, and statistics program for epidemiology on microcomputers. Atlanta, Georgia, USA; CDC, 1994.Google Scholar
26.van der Giessen, JWB, Haring, RM, Vauclare, E, Eger, A, Haagsma, J, van der Zeijst, BAM. Evaluation of the abilities of three diagnostic tests based on the polymerase chain reaction to detect Mycobacterium paratuberculosis in cattle; their application in a control program. J Clin Microbiol 1992; 30: 1216–19.CrossRefGoogle Scholar
27.Whipple, DL, Kapke, PA, Anderson, PR. Comparison of a commercial DNA probe test and three cultivation procedures for detection of Mycobacterium paratuberculosis in bovine feces. J Vet Diagn Invest 1992; 4: 23–7.CrossRefGoogle ScholarPubMed
28.Whitlock, RH, Rosenberger, AE. Faecal culture for Mycobacterium paratuberculosis, a recommended procedure. In: Proc 94th Ann Meeting US Animal Health Assn, Denver, 1990; 280–5.Google Scholar
29.Merkal, RS, Larsen, AB, Booth, GD. Analysis of the effects of inapparent bovine paratuberculosis. Am J Vet Res 1975; 36: 837–8.Google ScholarPubMed
30.Buergelt, CD, Duncan, JR. Age and milk production data of cattle culled from a dairy herd with paratuberculosis. JAVMA 1978; 173: 478–80.Google ScholarPubMed
31.Taylor, AW. Further observations on the incidence of infection with M. johnei in cattle. Vet Rec 1952; 64: 603–5.Google Scholar
32.Rankin, JD. The presence of Mycobacterium johnei in apparently normal cattle. Vet Rec 1954; 66: 550–1.Google Scholar