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The adsorption of endotoxin molecule in a microporous polyethylene hollow fibre membrane

Published online by Cambridge University Press:  19 October 2009

Yosuke Sawada ,
Reiko Fujii ,
Ikuo Igami ,
Atsushi Kawai ,
Teruo Kamiki  and
Makoto Niwa
Yosuke Sawada
Affiliation:
Product and Development Centre, Mitsubishi Rayon Co., Ltd, Higashiku, Nagoya 461, Japan
Reiko Fujii
Affiliation:
Product and Development Centre, Mitsubishi Rayon Co., Ltd, Higashiku, Nagoya 461, Japan
Ikuo Igami
Affiliation:
Product and Development Centre, Mitsubishi Rayon Co., Ltd, Higashiku, Nagoya 461, Japan
Atsushi Kawai
Affiliation:
Product and Development Centre, Mitsubishi Rayon Co., Ltd, Higashiku, Nagoya 461, Japan
Teruo Kamiki
Affiliation:
Public Health Research Institute of Kobe City, Chuoku, Kobe 650, Japan
Makoto Niwa
Affiliation:
Osaka City University Medical School, Department of Bacteriology, Abenoku, Osaka 545, Japan
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Summary

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The microporous polyethylene hollow fibre membrane is capable of adsorbing small-sized lipopolysaccharides (LPS) prepared by sonication dispersion, column chromatography on Sephadex G-75 and filtration through a filter membrane with a nominal pore size of 0·025 μg. Small-sized LPS had a one-thousandth of endotoxin activity as compared to intact LPS, when determined by the Synthetic Chromogenic Substrate method of LAL with a specific endotoxin activity of 73·7 ng/μg LPS. Fluorescent microscopy of fluorescein conjugated LPS on a microporous polyethylene hollow fibre showed that fiuorescein-LPS was adsorbed through the entire depth of the membrane texture. Accordingly the adsorption capacity of the filter for small-sized LPS was determined as 1·65 mg LPS/3·68 m2 surface/116 mg fibre/module.

Type
Research Article
Information
Journal of Hygiene , Volume 97 , Issue 1 , August 1986 , pp. 91 - 102
Copyright
Copyright © Cambridge University Press 1986

References

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