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Adherence to HEp-2 cells and enteropathogenic potential of Aeromonas spp.

Published online by Cambridge University Press:  19 October 2009

P. A. Grey  and
S. M. Kirov
P. A. Grey
Affiliation:
Department of Pathology, University of Tasmania Clinical School, Hobart, Tasmania 7000, Australia
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Summary

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Aeromonas strains (total = 60) of clinical, water and food origin were tested for adherence to HEp-2 cells. Environmental strains were selected (except for A. caviae) to include primarily those expressing other virulence-associated properties. Adhesion was markedly species-dependent (A. veronii biotype sobria, 15 of 20 [58%], A. caviae, 4 of 12 [33%] and A. hydrophila, 2 of 8 [11%]). A. veronii biotype sobria were adhesive, irrespective of source (62 and 54% for clinical and environmental strains, respectively). Adherent strains of this species were enterotoxin-positive and most (13 of 15) grew at 43 °C. A. caviae isolated from clinical specimens contained a higher proportion (75%) of adherent strains than environmental strains (13%). Virulent subsets of A. veronii biotype sobria and A. caviae are adherent to HEp-2 cells. The HEp-2 assay is a useful model for investigating mechanisms of adherence and enteropathogenicity of virulent Aeromonas species.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 2 , April 1993 , pp. 279 - 287
Copyright
Copyright © Cambridge University Press 1993

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