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Acquisition of genes from an O18:K1:H7 ColV+ strain of Escherichia coli renders intracranially-inoculated E. coli K12 highly virulent for chickens, ducks and guinea-pigs but not mice

Published online by Cambridge University Press:  19 October 2009

H. Williams Smith  and
M. B. Huggins
H. Williams Smith
Affiliation:
Houghton Poultry Research Station, Houghton, Huntingdon, Cambridgeshire, PE17 2DA
M. B. Huggins
Affiliation:
Houghton Poultry Research Station, Houghton, Huntingdon, Cambridgeshire, PE17 2DA
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Summary

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The virulence of intracranially-inoculated mutant forum of an O18ac:K1:H7 ColV+ stain of Escherichia coli (designated MW) that lacked different combinations of its O and K antigens and CoIV, and of an E. coli K12 strain to which these characters had been transmitted was studied in mice, chickens, ducks and guinea-pigs.

The O18+ K1+Co1V+ form of MW was highly virulent for chickens and mice hut the corresponding form of K12 was only highly virulent for chickens; the O18-K1-ColV- froms of both strains were of low virulence for chickens and mice. K1 was more important than O18 or ColV in determining virulence for both animal species, Ducks and guinca-pigs resembled chickens, not mice, in their response to infection with the O18+K1+ColV+ from of K12.

Pathogenesis studies revealed than the virulence of the forms of MW and K12 was associated with their ability to proliferate in the central nervous system; only low numbers of organisms were found in the blood and spleen of inoculated animals.

The O18+K1+ColV+ form of K12 multiplied in mouse brian and in mouse blood in eitro; its multiplication in chicken blood was partinlly inhibited. Agglutinins to this and other forms of K12 were found in chicken serum but not in mouse serum. Large doses of mouse serum given to chickens and large doeses of checken serum given to mice did not alter the manner in which these animals responded to K12 O18+K1+ColV+ infection. Vaceination protected chickens and mice against lethal intraeranial infection with the O18+K1+ColV+ forms of K12 or MW; it produicted a much stronger immunity in mice against intrapertoneal challenge than against intraeranial challeage

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 2 , October 1985 , pp. 363 - 374
Copyright
Copyright © Cambridge University Press 1985

References

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