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The 6-hour soluble antigen production test for comparing the infectivity of influenza virus preparations

Published online by Cambridge University Press:  15 May 2009

N. B. Finter  and
A. J. Beale
N. B. Finter
Affiliation:
Public Health Laboratory, Northampton
A. J. Beale
Affiliation:
Public Health Laboratory, Northampton
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1. The 6 hr. soluble antigen production test for comparing the infectivity of influenza virus preparations is described in detail. Dilutions of the preparations are inoculated intra-allantoically into groups of fertile eggs, and after 6 hr. of incubation the amounts of soluble antigen formed in their chorio-allantoic membranes are determined. Reproducible results can be obtained.

2. The similar test, in which haemagglutinins in the chorio-allantoic membranes are measured 6 hr. after inoculation of seed virus, is less accurate and less sensitive.

3. Eggs of different ages of incubation form different amounts of soluble antigen in their chorio-allantoic membranes in 6 hr. when inoculated with the same amounts of virus.

Type
Research Article
Information
Journal of Hygiene , Volume 54 , Issue 1 , March 1956 , pp. 58 - 67
Copyright
Copyright © Cambridge University Press 1956

References

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