Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-26T21:41:51.623Z Has data issue: false hasContentIssue false
  • English
  • Français

RESEARCH ARTICLE: Measurement Reproducibility of the Bioscan™ Flow-Through Respirometer Applied as a Toxicity-Based Early Warning System for Water Contamination

Published online by Cambridge University Press:  03 April 2007

Chris G. Campbell ,
Michelle M. Mascetti ,
William Hoppes  and
William T. Stringfellow
Chris G. Campbell
Affiliation:
Water Guidance and Monitoring Group, Environmental Protection Department, Lawrence Livermore National Laboratory, Livermore, California
Michelle M. Mascetti
Affiliation:
Water Guidance and Monitoring Group, Environmental Protection Department, Lawrence Livermore National Laboratory, Livermore, California
William Hoppes
Affiliation:
Water Guidance and Monitoring Group, Environmental Protection Department, Lawrence Livermore National Laboratory, Livermore, California
William T. Stringfellow
Affiliation:
Earth Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California
Get access

Abstract

It is crucial to understand the response reproducibility of on-line continuous-flow respirometers applied as biological toxicity monitors to alert plant operators to contamination in wastewater influent. Used as upset early warning systems, on-line respirometers could identify toxic waste streams that should be diverted from activated sludge treatment systems. This study used a continuous flow-through respirometer (Bioscan™) to examine the reproducibility in the respiration response of activated sludge biomass from a local wastewater treatment plant and a commercially available seed (POLYTOX®), when each was exposed to a toxic pH 3.0 buffer solution. Respiration response is characterized in this study as a change in dissolved oxygen concentration over time in the respirometer effluent. This response was segregated into groups, including within an inoculated population of a given seed source (activated sludge biomass or POLYTOX®), between different inoculations of the same seed source, and by the two different seed sources. Reproducibility in the response of the Bioscan™ respirometer was as high as 22% within populations, 23% between populations, and 29% for the same population over time. In addition, time dependency in the respirometer response to the toxin was examined. It was demonstrated that response to the same toxic pulse varies between and within populations and that reduced sensitivity of the microorganisms can occur if repeatedly exposed to an individual toxin. A genetic DGGE analysis for one population suggests that the observed reduction in sensitivity may be caused by changes in species composition.

Type
FEATURES & REVIEWS
Information
Environmental Practice , Volume 9 , Issue 1 , March 2007 , pp. 42 - 53
Copyright
© 2007 National Association of Environmental Professionals

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Campbell, C. G., M. Mascetti, R. Vellinger, and W. Hoppes. 2004. Testing A Flow-Through Respirometer for Early Warning of Water Contamination. Environmental Practice 6(4):306–315.Google Scholar
Cao, X., X. Liu, and X. Dong. 2003. Alkaliphilus crotonatoxidans sp. nov., A Strictly Anaerobic, Crotonate-Dismutating Bacterium Isolated from A Methanogenic Environment. International Journal of Systematic and Evolutionary Microbiology 53:971–975.Google Scholar
Chan, C.-M., W. H. Lo, K.-Y. Wang, and W.-F. Cheng. 1999. Monitoring the Toxicity of Phenolic Chemicals to Activated Sludge Using a Novel Optical Scanning Respirometer. Chemosphere 39(9):1421–1432.Google Scholar
Fielden, P. R., T. McCreedy, R. D. Snook, and B. J. T. Brown. 1996. On-Line Kinetic Measuring of Water Toxicity Using the Photobacterium, Vibrio fischeri. Analytical Communications 33:335–338.Google Scholar
Fisher Scientific. 1994. MSDS Buffer Solution pH 3.0. Fair Lawn, NJ.
InterLab. 2005. POLYTOX® Rapid Toxicity Test Technical Data Sheet. Technical Service Department, InterLab®, The Woodlands, TX.
King, E. F., and B. J. Dutka. 1986. Respirometric Techniques. In Toxicity Testing Using Microorganisms, volumes I & II, CRC Press, Boca Raton, FL, 75–113.
Kungolos, A. 2005. Evaluation of Toxic Properties of Industrial Wastewater Using On-Line Respirometry. Journal of Environmental Science and Health 40:869–880.Google Scholar
Lamba, P., and D. A. Webster. 1980. Effect of Growth Conditions on Yield and Heme Content of Vitreoscilla. Journal of Bacteriology 142(1):169–173.Google Scholar
Liu, J., and B. Mattiasson. 2002. Microbial BOD Sensors for Wastewater Analysis. Water Research 36:3786–3802.Google Scholar
Love, N. G., and C. B. Bott. 2000. A Review and Needs Survey of Upset Early Warning Devices. Project 99-WWF-2. Water Environmental Foundation, Alexandria, VA.
Mahendraker, V., and T. Viraraghavan. 1995. Respirometry in Environmental Engineering. Journal of Environmental Science and Health A30(4):713–734.Google Scholar
Maidak, B. L., J. R. Cole, T. G. Lilburn, C. T. Parker, P. R. Saxman, R. J. Farris, G. M. Garrity, G. J. Olsen, T. M. Schmidt, and J. M. Tiedje. 2001. The RDP-II (Ribosomal Database Project). Nucleic Acids Research 29:173–174.Google Scholar
Muyzer, G., E. C. de Waal, and A. G. Uitterlinden. 1993. Profiling of Complex Microbial Populations by Denaturing Gradient Gel Electrophoresis Analysis of Polymerase Chain Reaction-Amplified Genes Coding for 16S rRNA. Applied and Environmental Microbiology 59:695–700.Google Scholar
Reeves, J. B. 1976. Activated Sludge System Influent Toxicity Monitoring through Utilization of a Commercial, Continuous Respirometer. MS Thesis, Virginia Polytechnic Institute and State University, Blacksburg, VA.
Ren, E., and P. D. Frymier. 2003. Toxicity Estimation of Phenolic Compounds by Bioluminescent Bacterium. Journal of Environmental Engineering 129(4):328–35.Google Scholar
Rozzi, A., E. Ficara, C. M. Cellamare, and G. Bortone. 1999. Characterization of Textile Wastewater and Other Industrial Wastewaters by Respirometric and Titration Biosensors. Water Science and Technology 40(1):161–168.Google Scholar
Weisburg, W. G., S. M. Barns, D. A. Pelletier, and D. J. Lane. 1991. 16S Ribosomal DNA Amplification for Phylogenetic Study. Journal of Bacteriology 173:697–703.Google Scholar