Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-23T17:11:45.605Z Has data issue: false hasContentIssue false
  • English
  • Français

Soil persistence of DNA from transgenic poplar

Published online by Cambridge University Press:  02 July 2009

Martina Bonadei ,
Alma Balestrazzi ,
Barbara Frigerio  and
Daniela Carbonera
Martina Bonadei
Affiliation:
Dipartimento di Genetica e Microbiologia, Università di Pavia, via Ferrata 1, 27100 Pavia, Italy
Alma Balestrazzi
Affiliation:
Dipartimento di Genetica e Microbiologia, Università di Pavia, via Ferrata 1, 27100 Pavia, Italy
Barbara Frigerio
Affiliation:
Dipartimento di Genetica e Microbiologia, Università di Pavia, via Ferrata 1, 27100 Pavia, Italy
Daniela Carbonera
Affiliation:
Dipartimento di Genetica e Microbiologia, Università di Pavia, via Ferrata 1, 27100 Pavia, Italy

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The presence of recombinant DNA in soil cultivated with white poplars (Populus alba L.) expressing either the bar transgene for herbicide tolerance or the StSy transgene for resveratrol production, respectively, was investigated in a greenhouse over a 20-month period. The bar trial included the transgenic lines 5P56 and 6EA22P56 and the untransformed line, while the StSy trial was established with the transgenic lines 5EAC1 and 12EAC1 and with the untransformed line. All the transgenic poplars harbored the nptII marker gene. Plantlets were cultivated in pots, and soil samples were mixed in order to obtain composite pools which were used for molecular analyses. The 35SCaMV-bar (1504 bp), 35SCaMV-StSy (1403 bp) and NosP-nptII (1188 bp) sequences were detected in total DNA extracted from soil samples taken at different times after planting, using PCR/Southern blot hybridization. Microcosm experiments, carried out to assess the effects of temperature and DNA purity on transgene persistence, revealed only a partial correlation between the intensity of hybridization signals and the parameters tested.

Keywords

greenhouse trialmicrocosmPopulus albarecombinant DNAsoil persistence
Type
Research Article
Information
Environmental Biosafety Research , Volume 8 , Issue 2 , April 2009 , pp. 79 - 86
Copyright
© ISBR, EDP Sciences, 2009

References

Alhamd, L, Arakaki, S, Hagihara, A (2004) Decomposition of leaf litter of four tree species in a subtropical evergreen broad-leaved forest, Okinawa Island, Japan. For. Ecol. Man. 202: 1–11 CrossRef
Balestrazzi A, Allegro G, Confalonieri M (2006) Genetically modified trees expressing genes for insect pest resistance. In Fladung M, Ewald D, eds, Tree Transgenesis: Recent Developments, Springer-Verlag, Berlin, Heidelberg, pp 253–273
Balestrazzi, A, Bonadei, M, Carbonera, D (2007) Nuclease-producing bacteria in soil cultivated with herbicide resistant transgenic white poplars. Ann. Microbiol. 57: 531–536 CrossRef
Blum, SAE, Lorenz, MG, Wackernagel, W (1997) Mechanism of retarded DNA degradation and prokaryotic origin of DNases in non sterile soils. System. Appl. Microbiol. 20: 513–521 CrossRef
Ceccherini, MT, Poté, J, Kay, E, Van, V, Marechal, J, Pietramellara, G, Nannipieri, P, Vogel, TM, Simonet, P (2003) Degradation and transformability of DNA from transgenic leaves. Appl. Environ. Microbiol. 69: 673–678 CrossRef
Chander, K, Goyal, S, Kapoor, KK (1995) Microbial biomass dynamics during the decomposition of leaf litter of poplar and eucalyptus in a sandy loam. Biol. Fertil. Soils 19: 269–279 CrossRef
Confalonieri M, Belenghi B, Balestrazzi A, Negri S, Facciotto G, Schenone G, DelleDonne M (2000) Transformation of elite white poplar (P. alba) cv `Villafranca' and evaluation of herbicide resistance. Plant Cell Rep. 19: 978–982
Cotrufo MF, De Angelis P, Polle A (2005) Leaf litter production and decomposition in a poplar short-rotation coppice exposed to free air CO2 enrichment (POPFACE). Global Change Biol. 11: 971–982
de Vries, J, Heine, M, Harms, K, Wackernagel, W (2003) Spread of recombinant DNA by roots and pollen of transgenic potato plants, identified by highly specific biomonitoring using natural transformation of an Acinetobacter sp. Appl. Environ. Microbiol. 69: 4455–4462 CrossRef
England, LS, Vincent, ML, Trevors, JT, Holmes, SB (2004) Extraction, detection and persistence of extracellular DNA in forest litter microcosms. Mol. Cell. Probes 18: 313–319 CrossRef
Gebhard, F, Smalla, K (1999) Monitoring field releases of genetically modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer. FEMS Microbiol. Ecol. 28: 261–272 CrossRef
Giorcelli A, Sparvoli F, Mattivi F, Balestrazzi A, Tava A, Vrhovsek U, Bollini R, Confalonieri M (2004) Expression of stilbene synthase (StSy) gene from grapevine in transgenic white poplar results in high accumulation of the antioxidant compounds resveratrol glucosides. Trans. Res. 13: 203–214
Hay, I, Morency, M-J, Seguin, A (2002) Assessing the persistence of DNA in decomposing leaves of genetically modified poplar trees. Can. J. For. Res. 32: 977–982 CrossRef
Hobbie, SE, Reich, PB, Oleksyn, J, Ogdahl, M, Zytkowiak, R, Hale, C, Karolewski, P (2006) Tree species effects on decomposition and forest floor dynamics in a common garden. Ecology 87: 2288–2297 CrossRef
Keese, P (2008) Risks from GMOs due to Horizontal Gene Transfer. Environ. Biosafety Res. 7: 123–149 CrossRef
Krsek, M, Wellington, EMH (1999) Comparison of different methods for the isolation and purification of total community DNA from soil. J. Microbiol. Methods 39: 1–16 CrossRef
Lerat, S, England, LS, Vincent, ML, Pauls, KP, Swanton, CJ, Klironomos, JN, Trevors, JT (2005) Real-time polymerase chain reaction quantification of the transgenes for Roundup Ready corn and Roundup Ready soybean in soil samples. J. Agric. Food Chem. 53: 1337–1342 CrossRef
Lilley, AK, Bailey, MJ, Barr, M, Kilshaw, K, Timms-Wilson, TM, Day, MJ, Norris, SJ, Jones, TH, Godfray, HCJ (2003) Population dynamics and gene transfer in genetically modified bacteria in a model microcosm. Mol. Ecol. 12: 3097–3107 CrossRef
Luo, Y, Wan, S, Hui, D, Wallace, LL (2001) Acclimatization of soil respiration to warming in a tall grass praire. Nature 413: 622–625 CrossRef
Monier J-M, Bernillon D, Kay E, Faugier A, Rybalka O, Dessaux Y, Simonet P, Vogel TM (2007) Detection of potential transgenic plant DNA recipients among soil bacteria. Environ. Biosafety Res. 6: 71–83
Pontiroli, A, Simonet, P, Frostegard, A, Vogel, TM, Monier, J-M (2007) Fate of transgenic plant DNA in the environment. Environ. Biosafety Res. 6: 15–35 CrossRef
Richter, B, Smalla, K (2007) Screening of rhizosphere and soil bacteria for transformability. Environ. Biosafety Res. 6: 91–99 CrossRef
Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues, Gelvin SB, Schilperoort RA, eds, Plant Molecular Biology Manual, Kluwer, Dordrecht, pp A6, 1–10
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Sparvoli, F, Martin, C, Scienza, A, Gavazzi, G, Tonelli, C (1994) Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.). Plant Mol. Biol. 24: 743–755 CrossRef
Thompson, CJ, Movva, NR, Tizard, R, Crameri, R, Davies, JE, Lawereyes, M, Botterman, J (1987) Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO J. 6: 2519–2523
Van Overbeek, L, Ray, J, Van Elsas, JD (2007) Assessment of transformability of bacteria associated with tomato and potato plants. Environ. Biosafety Res. 6: 85–89 CrossRef
Widmer, F, Seidler, RJ, Donegan, KK, Reed, GL (1997) Quantification of transgenic plant marker gene persistence in the field. Mol. Ecol. 6: 1–7 CrossRef
Zelasco, S, Reggi, S, Calligari, P, Balestrazzi, A, Bongiorni, C, Quattrini, E, Delia, G, Bisoffi, S, Fogher, C, Confalonieri, M (2006) Expression of the Vitreoscilla hemoglobin (VHb)-encoding gene in transgenic white poplar: plant growth and biomass production, biochemical characterization and cell survival under submergence, oxidative and nitrosative stress conditions. Mol. Breed. 17: 201–216 CrossRef
Zelasco, S, Ressegotti, V, Confalonieri, M, Carbonera, D, Calligari, P, Bonadei, M, Bisoffi, S, Yamada, K, Balestrazzi A (2007) Evaluation of MAT-vector system in white poplar (Populus alba L.) and production of ipt marker-free transgenic plants by `singlestep transformation'. Plant Cell Tiss. Organ. Cult. 91: 61–72 CrossRef
Zhou, JZ, Bruns, MA, Tiedje, JM (1996) DNA recovery from soils of diverse composition. Appl. Environ. Microbiol. 62: 316–322