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In-field frequencies and characteristics of oilseed rape with double herbicide resistance

Published online by Cambridge University Press:  10 July 2009

Antje Dietz-Pfeilstetter  and
Peter Zwerger
Antje Dietz-Pfeilstetter
Affiliation:
Institute for Biosafety of Genetically Modified Plants and Institute for Plant Protection in Field Crops and Grassland, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Messeweg 11-12, 38104 Braunschweig, Germany
Peter Zwerger
Affiliation:
Institute for Biosafety of Genetically Modified Plants and Institute for Plant Protection in Field Crops and Grassland, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Messeweg 11-12, 38104 Braunschweig, Germany

Abstract

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When growing different transgenic herbicide-resistant oilseed rape cultivars side by side, seeds with multiple herbicide resistance can arise, possibly causing problems for the management of volunteer plants. Large-scale field experiments were performed in the years 1999/2000 and 2000/2001 in order to investigate the frequencies and the consequences of the transfer of herbicide resistance genes from transgenic oilseed rape to cultivars grown on neighboring agricultural fields. Transgenic oilseed rape with resistance to glufosinate-ammonium (LibertyLink, LL) and with glyphosate resistance (RoundupReady, RR), respectively, was sown in adjacent 0.5 ha plots, surrounded by about 8 ha non-transgenic oilseed rape. The plots and the field were either in direct contact (0.5 m gap width) or they were separated by 10 m of fallow land. Seed samples taken during harvest in the transgenic plots at different distances were investigated for progeny with resistance to the respective other herbicide. It was found that outcrossing frequencies were reduced to different extents by a 10 m isolation distance. In addition to pollen-mediated transgene flow as a result of outcrossing, we found considerable seed-mediated gene flow by adventitious dispersal of transgenic seeds through the harvesting machine. Volunteer plants with double herbicide resistance emerging in the transgenic plots after harvest were selected by suitable applications of the complementary herbicides Basta® and Roundup Ultra®. In both years, double-resistant volunteers were largely restricted to the inner edges of the plots. Expression analysis under controlled laboratory conditions of double-resistant plants generated by manual crosses revealed stability of transgene expression even at elevated temperatures. Greenhouse tests with double-resistant oilseed rape plants gave no indication that the sensitivity to a range of different herbicides is changed as compared to non-transgenic oilseed rape.

Keywords

herbicide resistanceBrassica napusgene flowgene expressiongene stackingvolunteer control
Type
Research Article
Information
Environmental Biosafety Research , Volume 8 , Issue 2 , April 2009 , pp. 101 - 111
Copyright
© ISBR, EDP Sciences, 2009

References

Assaad, FF, Tucker, KL, Signer, ER (1993) Epigenetic repeat-induced gene silencing (RIGS) in Arabidopsis. Plant Mol. Biol. 22: 1067–1085 CrossRef
Beaujean, A, Sangwan, RS, Hodges, M, Sangwan-Norreel, BS (1998) Effect of ploidy and homozygosity on transgene expression in primary tobacco transformants and their androgenetic progenies. Mol. Gen. Genet. 260: 361–371 CrossRef
Becker, HC, Damgaard, C, Karlsson, B (1992) Environmental variation for outcrossing rate in rapeseed (Brassica napus). Theor. Appl. Genet. 84: 303–306
Beckie, HJ, Warwick, SI, Nair, H, Séguin-Swartz G (2003) Gene flow in commercial fields of herbicide-resistant canola (Brassica napus). Ecol. Appl. 13: 1276–1294 CrossRef
Beckie, HJ, Séguin-Swartz, G, Nair, H, Warwick, SI, Johnson E (2004) Multiple herbicide-resistant canola can be controlled by alternative herbicides. Weed Sci. 52: 152–157 CrossRef
Caligari, PDS, Yapabandara, YMHB, Paul, EM, Perret, J, Roger, P, Dunwell, JM (1993) Field performance of derived generations of transgenic tobacco. Theor. Appl. Genet. 86: 875–879 CrossRef
Cardina, J, Johnson, GA, Sparrow, DH (1997) The nature and consequences of weed spatial distribution. Weed Sci. 45: 364–373
Cousens, R, Croft, AM (2000) Weed populations and pathogens. Weed Res. 40: 63–82 CrossRef
Dietz-Pfeilstetter, A, Barg, E, Weber, A (2005) Gene expression in transgenic virus resistant and herbicide-resistant plants under greenhouse and under field conditions. Nachrichtenbl. Deut. Pflanzenschutzd. 57: 167–171
Downey RK (1999) Gene flow and rape – the Canadian experience. In Lutman PJW, ed, Gene Flow and Agriculture – Relevance for Transgenic Crops, British Crop Protection Council, Vol. 72, pp 109–116
Gruber, S, Pekrun, C, Claupein, W (2004) Population dynamics of volunteer oilseed rape (Brassica napus L.) affected by tillage. Eur. J. Agron. 20: 351–361 CrossRef
Hall, L, Topinka, K, Huffman, J, Davis, L, Good, A (2000) Pollen flow between herbicide-resistant Brassica napus is the cause of multiple-resistant B. napus volunteers. Weed Sci. 48: 688–694 CrossRef
Hüsken, A, Dietz-Pfeilstetter A (2007) Pollen-mediated intraspecific gene flow from herbicide resistant oilseed rape (Brassica napus L.). Transgenic Res. 16: 557–569 CrossRef
Ingram, J (2000) The separation distances required to ensure cross-pollination is below specified limits in non-seed crops of sugar beet, maize and oilseed rape. Plant Var. Seeds 13: 181–199
James, VA, Avart, C, Worland, B, Snape, JW, Vain, P (2002) The relationship between homozygous and hemizygous transgene expression levels over generations in populations of transgenic rice plants. Theor. Appl. Genet. 104: 553–561 CrossRef
Jørgensen, T, Hauser, TP, Jørgensen, RB (2007) Adventitious presence of other cultivars in oilseed rape (Brassica napus) from seed banks and certified seed. Seed Science Res. 17: 115–125 CrossRef
Knispel, AL, McLachlan, SM, Van Acker, RC, Friesen, LF (2008) Gene flow and multiple herbicide resistance in escaped canola populations. Weed Sci. 56: 72–80 CrossRef
Lutman, PJW, Berry, K, Payne, RW, Simpson, E, Sweet, JB, Champion, GT, May, MJ, Wightman, P, Walker, K, Lainsbury, M (2005) Persistence of seeds from crops of conventional and herbicide tolerant oilseed rape (Brassica napus). Proc. Royal Soc. B-Biol. Sci. 272: 1909–1915 CrossRef
Maiti, IB, Gowda, S, Kiernan, J, Ghosh, SK, Shepherd, RJ (1997) Promoter/leader deletion analysis and plant expression vectors with the figwort mosaic virus (FMV) full length transcript (FLt) promoter containing single or double enhancer domains. Transgenic Res. 6: 143–156 CrossRef
Matzke, MA, Neuhuber, F, Matzke, AJM (1993) A variety of epistatic interactions can occur between partially homologous transgene loci brought together by sexual crossing. Mol. Gen. Genet. 238: 379–386 CrossRef
Meyer, P, Saedler, H (1996) Homology-dependent gene silencing in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 23–48 CrossRef
Meyer, P, Linn, F, Heidmann, I, Meyer, ZAH, Niedenhof, I, Saedler, H (1992) Endogenous and environmental factors influence 35S promoter methylation of a maize A1 gene construct in transgenic petunia and its colour phenotype. Mol. Gen. Genet. 231: 345–352 CrossRef
Park, Y-D, Papp, I, Moscone, EA, Iglesias, VA, Vaucheret, H, Matzke, AJM, Matzke, MA (1996) Gene silencing mediated by promoter homology occurs at the level of transcription and results in meiotically heritable alterations in methylation and gene activity. The Plant J. 9: 183–194 CrossRef
Pekrun, C, Lutman, PJW, Baeumer, K (1997) Induction of secondary dormancy in rape seeds (Brassica napus L.) by prolonged imbibition under conditions of water stress or oxygen deficiency in darkness. Eur. J. Agron. 6: 245–255 CrossRef
Pfeilstetter, E, Matzk, A, Feldmann, SD, Schiemann, J (2000) Rapid and efficient screening of phosphinothricin tolerant oilseed rape (Brassica napus) with a novel germination test. Euphytica 113: 119–124 CrossRef
Price, JS, Hobson, RN, Neale, MA, Bruce, DM (1996) Seed losses in commercial harvesting of oilseed rape. J. Agricultural Engineering Research 65: 183–191 CrossRef
Rakow, G, Woods, DL (1987) Outcrossing in rape and mustard under Saskatchewan prairie conditions. Can. J. Plant Sci. 67: 147–151 CrossRef
Reboud, X (2003) Effect of a gap on gene flow between otherwise adjacent transgenic Brassica napus crops. Theor. Appl. Genet. 106: 1048–1058 CrossRef
Rew, LJ, Cousens, RD (2001) Spatial distribution of weeds in arable crops: are current sampling and analytical methods appropriate? Weed Res. 41: 1–18 CrossRef
Richins, RD, Scholthof, HB, Shepherd, RJ (1987) Sequence of figwort mosaic virus DNA (caulimovirus group). Nucl. Acids Res. 15: 8451–8466 CrossRef
Rieger, MA, Lamond, M, Preston, C, Powles, SB, Roush, RT (2002) Pollen-mediated movement of herbicide resistance between commercial canola fields. Science 296: 2386–2388 CrossRef
Schlink S (1998) 10 years survival of rape seed (Brassica napus L.) in soil. J. Plant Diseases Protection, Special Issue XVI: 169–172
Senior, IJ, Moyes, C, Dale, PJ (2002) Herbicide sensitivity of transgenic multiple herbicide-tolerant oilseed rape. Pest Manag. Sci. 58: 405–412 CrossRef
Simpson EC, Norris CE, Law JR, Thomas JE, Sweet JB (1999) Gene flow in genetically modified herbicide tolerant oilseed rape (Brassica napus) in the UK. In Lutman PJW, ed, Gene Flow and Agriculture – Relevance for Transgenic Crops, British Crop Protection Council, Vol. 72, pp 75–81
Staniland, BK, McVetty, PBE, Friesen, LF, Yarrow, S, Freyssinet, G, Freyssinet, M (2001) Effectiveness of border areas in confining the spread of transgenic Brassica napus pollen. Can. J. Plant Sci. 80: 521–526 CrossRef
Taberlet, P, Gielly, L, Pautou, G, Bouvet, J (1991) Universal primers for amplification of three noncoding regions of chloroplast DNA. Plant Mol. Biol. 17: 1105–1109 CrossRef
Tang J, Scarth R, Fristensky B (2003) Effects of genomic position and copy number of Acyl-ACP thioesterase transgenes on the level of the target fatty acids in Brassica napus L. Mol. Breed. 12: 71–81
Thierry, D, Vaucheret, H (1996) Sequence homology requirements for transcriptional silencing of 35S transgenes and post-transcriptional silencing of nitrite reductase (trans)genes by the tobacco 271 locus. Plant Mol. Biol. 32: 1075–1083 CrossRef
Tinker, NA, Fortin, MG, Mather, DE (1993) Random amplified polymorphic DNA and pedigree relationships in spring barley. Theor. Appl. Genet. 85: 976–984 CrossRef