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Hybridization between GM soybean (Glycine max (L.) Merr.) and wild soybean (Glycine soja Sieb. et Zucc.) under field conditions in Japan

Published online by Cambridge University Press:  08 October 2010

Aki Mizuguti ,
Kentaro Ohigashi ,
Yasuyuki Yoshimura ,
Akito Kaga ,
Yosuke Kuroda  and
Kazuhito Matsuo
Aki Mizuguti*
Affiliation:
National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan
Kentaro Ohigashi
Affiliation:
National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan
Yasuyuki Yoshimura
Affiliation:
National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan
Akito Kaga
Affiliation:
National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan
Yosuke Kuroda
Affiliation:
National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan Present address: National Agricultural Research Center for Hokkaido Region, National Agriculture and Food Research Organization, Memuro, Hokkaido, Japan
Kazuhito Matsuo
Affiliation:
National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan
*
* Corresponding author: [email protected]

Abstract

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Accumulation of information about natural hybridization between GM soybean (Glycine max) and wild soybean (Glycine soja) is required for risk assessment evaluation and to establish biosafety regulations in Japan. This is particularly important in areas where wild relatives of cultivated soybean are grown (i.e. East Asia including Japan). To collect information on temporal and spatial factors affecting variation in hybridization between wild and GM soybean, a two year hybridization experiment was established that included one wild soybean and five GM soybean cultivars with different maturity dates. Hybridization frequencies ranged from 0 to 0.097%. The maximum hybridization frequency (0.097%) was obtained from wild soybean crossed with GM soybean cv. AG6702RR, which were adjacently cultivated with wild soybean, with 25 hybrids out of 25 741 seedlings tested. Cultivar AG6702RR had the most synchronous flowering period with wild soybean. Ten hybrids out of 25 741 were produced by crossing with cv. AG5905RR, which had the second most synchronous flowering period with wild soybean. Most hybrids were found where GM and wild soybeans were adjacently cultivated, whereas only one hybrid was detected from wild soybean plants at 2 m, 4 m and 6 m from a pollen source (GM soybean). Differences in flowering phenology, isolation distance and presence of buffer plants accounted for half of the variation in hybridization frequency in this study. Temporal and spatial isolation will be effective strategies to minimize hybridization between GM and wild soybean.

Keywords

buffer plantdistanceflowering overlapflowering synchronism
Type
Research Article
Information
Environmental Biosafety Research , Volume 9 , Issue 1 , January 2010 , pp. 13 - 23
Copyright
© ISBR, EDP Sciences

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