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Effects of rhizodeposition of non-transgenic and transplastomic tobaccos on the soil bacterial community

Published online by Cambridge University Press:  03 April 2008

Lorenzo Brusetti ,
Aurora Rizzi ,
Alessandro Abruzzese ,
Gian Attilio Sacchi ,
Enzio Ragg ,
Marco Bazzicalupo ,
Claudia Sorlini  and
Daniele Daffonchio
Lorenzo Brusetti
Affiliation:
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche (DISTAM), Università degli Studi di Milano, via Celoria 2, 20133, Milano, Italy
Aurora Rizzi
Affiliation:
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche (DISTAM), Università degli Studi di Milano, via Celoria 2, 20133, Milano, Italy
Alessandro Abruzzese
Affiliation:
Dipartimento di Produzione Vegetale (DIPROVE), Università degli Studi di Milano, via Celoria 2, 20133, Milano, Italy
Gian Attilio Sacchi
Affiliation:
Dipartimento di Produzione Vegetale (DIPROVE), Università degli Studi di Milano, via Celoria 2, 20133, Milano, Italy
Enzio Ragg
Affiliation:
Dipartimento di Scienze Molecolari ed Agroalimentari (DISMA), Università degli Studi di Milano, via Celoria 2, 20133, Milano, Italy
Marco Bazzicalupo
Affiliation:
Dipartimento di Biologia Animale e Genetica (DBAG), Università degli Studi di Firenze, via Romana 17, 50125, Firenze, Italy
Claudia Sorlini
Affiliation:
Dipartimento di Biologia Animale e Genetica (DBAG), Università degli Studi di Firenze, via Romana 17, 50125, Firenze, Italy
Daniele Daffonchio
Affiliation:
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche (DISTAM), Università degli Studi di Milano, via Celoria 2, 20133, Milano, Italy

Abstract

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The effect of root-released compounds of transplastomic tobacco (Nicotiana tabacum) on the soil bacterial community structure, and their potential to support horizontal gene transfer (HGT) to bacteria have been studied. Soil microcosms were exposed to root-released compounds collected from transplastomic and non-transgenic tobacco cultivars. Cluster analysis of automated ribosomal intergenic spacer analysis (ARISA) profiles of the soil bacterial community after 48 h incubation grouped the transgenic cultivar apart from the non-transgenic, indicating that it had a rhizodeposition pattern different from the parental plants. However, these differences were less than between the two non-transgenic tobacco cultivars studied. NMR characterization of the root-released compounds showed some differences in chemical fingerprinting pattern between the transplastomic and the parental cultivar. However, the effect on bacterial community structure was transient, and tended to disappear after 96 h of incubation. The potential of root-released compounds as a source of transforming DNA for bacteria was investigated by using four potential recipient species. No transformants were obtained following exposure of all the recipients to the root-released compounds. Root-released compounds amended to transgene donor DNA decreased the transformation frequency of Acinetobacter baylyistrain ADP1200, while Azospirillum, Agrobacterium, and Sinorhizobium strains failed to develop competence also in the presence of an external added transgene source. Detection of plastid sequences by PCR suggested that a very low amount of fragmented plastid donor DNA was present in the root-released compounds.

Keywords

transplastomic tobaccoroot-released compoundssoil bacterial diversityhorizontal gene transferAcinetobacterroot exudate composition
Type
Research Article
Information
Environmental Biosafety Research , Volume 7 , Issue 1 , January 2008 , pp. 11 - 24
Copyright
© ISBR, EDP Sciences, 2008

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