Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-26T04:11:53.687Z Has data issue: false hasContentIssue false
  • English
  • Français

Diabrotica-resistant Bt-maize DKc5143 event MON88017 has no impact on the field densities of the leafhopper Zyginidia scutellaris

Published online by Cambridge University Press:  03 February 2011

Stefan Rauschen ,
Eva Schultheis ,
Heinz Hunfeld ,
Frank Schaarschmidt ,
Ingolf Schuphan  and
Sabine Eber
Stefan Rauschen*
Affiliation:
RWTH Aachen University, Department of Plant Physiology (Biology III), Worringerweg 1, 52074 Aachen, Germany
Eva Schultheis
Affiliation:
RWTH Aachen University, Department of Plant Physiology (Biology III), Worringerweg 1, 52074 Aachen, Germany
Heinz Hunfeld
Affiliation:
Institute for Applied Biotechnology in the Tropics at the University of Göttingen, 37077 Göttingen, Germany
Frank Schaarschmidt
Affiliation:
Leibniz Universität Hannover, Faculty of Natural Sciences, Institute of Biostatistics, Herrenhaeuser Straße 2, 30419 Hannover, Germany
Ingolf Schuphan
Affiliation:
RWTH Aachen University, Institute of Environmental Research (Biology V), Worringerweg 1, 52074 Aachen, Germany
Sabine Eber
Affiliation:
RWTH Aachen University, Department of Plant Physiology (Biology III), Worringerweg 1, 52074 Aachen, Germany
*
*Corresponding author: [email protected]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Auchenorrhyncha (planthoppers and leafhoppers) are herbivorous organisms that can ingest Cry proteins from genetically engineered Bt-crops depending on their feeding behaviour. Consequently, they might be directly affected by non-target Bt-protein action and more importantly serve as a source of Cry protein exposure to beneficial predatory arthropods. During a three year field study, we surveyed the community of Auchenorrhyncha in Diabrotica-resistant Bt-maize DKc5143-Bt (event MON88017), its near-isogenic line and two conventional hybrids using sweep netting and custom made sticky traps. Zyginidia scutellaris (Herrich-Schäffer) (Hemiptera: Cicadellidae) represented more than 60% of all captured individuals, indicating that it is the dominant leafhopper within the maize community. The statistical analysis of Z. scutellaris data using confidence intervals for the ratios of mean abundance showed no consistent differences between the Bt-maize and the near-isogenic cultivar, indicating no negative impact of event MON88017. The two conventional hybrids Benicia and DK315 exhibited differences in terms of Z. scutellaris densities, which were greater than those observed between MON88017 and the near-isogenic line, but also not consistent over the years. Six more species accounted for an additional 39% of all captured specimens, while ten more species were found only as single individuals and can be considered vagrants from neighbouring habitats. These results inform future field work on the non-target impact of Bt-maize on this group of arthropods and monitoring approaches to assess biological control function by surveying herbivore communities.

Keywords

Bt-maizeenvironmental risk assessmentMON88017non-target organismsZyginidia scutellaris
Type
Research Article
Information
Environmental Biosafety Research , Volume 9 , Issue 2 , April 2010 , pp. 87 - 99
Copyright
© ISBR, EDP Sciences, 2011

References

Árpás, K, Tóth, F, Kiss, J (2005) Foliage-dwelling arthropods in Bt-transgenic and isogenic maize: a comparison through spider web analysis. Acta Phytopathol. Entomol. Hung. 40: 347–353 Google Scholar
Biedermann R, Niedringhaus R (2004) Die Zikaden Deutschlands. Bestimmungstafeln für alle Arten. Wissenschaftlich Akademischer Buchvertrieb Fründ, Scheeßel
Candolfi, MP, Brown, K, Grimm, C, Reber, B, Schmidli, H (2004) A faunistic approach to assess potential side-effects of genetically modified Bt-corn on non-target arthropods under field conditions. Biocontrol. Sci. Techn. 14: 129–170 Google Scholar
Dutton, A, Obrist, L, D’Alessandro, M, Diener, L, Müller, M, Romeis, J, Bigler, F (2004) Tracking Bt-toxin in transgenic maize to assess the risks on non-target arthropods. IOBC/WPRS Bulletin 27: 57–63 Google Scholar
EFSA (2006) Guidance document of the scientific panel on genetically modified organisms for the risk assessment of genetically modified plants and derived food and feed. The EFSA Journal 99: 1–100
EU (2001) Directive 2001/18/EC of the European Parliament and of the Council of 12 March 2001 on the deliberate release into the environment of genetically modified organisms and repealing Council Directive 90/220/EEC. L 106/1 of 4/17/2001
EU (2002) Council Decision of 3 October 2002 establishing guidance notes supplementing Annex VII to Directive 2001/18/EC of the European Parliament and of the Council on the deliberate release into the environment of genetically modified organisms and repealing Council Directive 90/220/EEC. L 280/27 of 10/18/2002
Glare TR, O’Callaghan M (2000) Bacillus thuringiensis: Biology, ecology and safety. Chichester, New York, Weinheim, Brisbane, Singapore, Toronto, John Wiley & Sons Limited
Head, G, Brown, CR, Groth, ME, Duan, JJ (2001) Cry1Ab protein levels in phytophagous insects feeding on transgenic corn: implications for secondary exposure risk assessment. Entomol. Exp. Appl. 99: 37–45 Google Scholar
Hothorn T, Bretz F, Westfall P, Heiberger RM (2007) Multcomp: simultaneous inference for general linear hypotheses. R package version 0.991-9
Hothorn, T, Bretz, F, Westfall, P (2008) Simultaneous inference in general parametric models. Biometrical J. 50: 346–363 Google ScholarPubMed
Hummel, HE (2003) Introduction of Diabrotica virgifera virgifera into the Old World and its consequences: a recently acquired invasive alien pest species on Zea mays from North America. Comm. Agr. Appl. Biol. Sci. 68: 45–57 Google ScholarPubMed
James C (2009) Global status of commercialized biotech/GM crops: 2009. ISAAA Briefs 41, ISAAA, Ithaca
Kiss J, Szentkirályi F, Tóth F, Szénási A, Kádár F, Árpás K, Szekeres D, Edwards CR (2002) Bt corn: impact on non-targets and adjusting to local IPM systems. In Lelley T, Balázs E, Tepfer M, eds, Ecological Impact of GMO Dissemination in Agro-Ecosystems Wien, Facultas, pp 157–172
Kiss J, Edwards CR, Berger HK, Cata P, Cean M, Cheek S, Derron J, Festic H, Furlan L, Igrc-Barcic J, Ivanova I, Lammers W, Omelyuta V, Princzinger G, Reynaud P, Sivcev I, Sivicek P, Urek G, Vahala O (2005) Monitoring of Western Corn Rootworm (Diabrotica virgifera virgifera LeConte) in Europe 1992–2003. In Vidal S, Kuhlmann U, Edwards CR, eds, Western Corn Rootworm: Ecology and Management, Wallingford, CABI Publishing, pp 29–39
McCann, MC, Trujillo, WA, Riordan, SG, Sorbet, R, Bogdanova, NN, Sidhu, RS (2007) Comparison of the forage and grain composition from insect-protected and glyphosate-tolerant MON88017 corn to conventional corn (Zea mays L.). J. Agric. Food Chem. 55: 4034–4042 Google Scholar
McCullagh P, Nelder JA (1989) Generalized Linear Models. Boca Raton, Chapman & Hall/CRC
McCulloch CE, Searle SR (2001) Generalized, Linear and Mixed Models. NewYork, John Wiley & Sons Inc
Meissle, M, Romeis, J (2009) The web-building spider Theridion impressum (Araneae: Theridiidae) is not adversely affected by Bt maize resistant to corn rootworms. Plant Biotech. J. 7: 645–656 Google Scholar
Miller, N, Estoup, A, Toepfer, S, Bourguet, D, Lapchin, L, Derridj, S, Kim, KS, Reynaud, P, Furlan, L, Guillemaud, T (2005) Multiple transatlantic introductions of the Western Corn Rootworm. Science 310: 992 Google ScholarPubMed
Nickel H (2003) The Leafhoppers and Planthoppers of Germany (Hemiptera, Auchenorrhyncha), patterns and strategies in a highly diverse group of phytophagous insects. Sofia, Moscow, Pensoft Series Faunistica
Obrist, LB, Dutton, A, Albajes, R, Bigler, F (2006) Exposure of arthropod predators to Cry1Ab toxin in Bt maize fields. Ecol. Entomol. 31: 143–154 Google Scholar
Pons, X, Lumbierres, B, López, C, Albajes, R (2005) Abundance of non-target pests in transgenic Bt-maize: a farm scale study. Eur. J. Entomol. 102: 73–79 Google Scholar
R Development Core Team (2008) R: A language and environment for statistical computing. R Foundation for Statistical Computing. Vienna, http://www.R-project.org
Raps, A, Kehr, J, Gugerli, P, Moar, WJ, Bigler, F, Hilbeck, A (2001) Immunological analysis of phloem sap of Bacillus thuringiensis corn and of the nontarget herbivore Rhopalosiphum padi (Homoptera: Aphididae) for the presence of Cry1Ab. Mol. Ecol. 10: 525–533 Google ScholarPubMed
Rauschen, S, Eckert, J, Schaarschmidt, F, Schuphan, I, Gathmann, A (2008) An evaluation of methods for assessing the impacts of Bt-maize MON810 cultivation and pyrethroid insecticide use on Auchenorrhyncha (Planthoppers and Leafhoppers). Agric. For. Entomol. 10: 331–339 Google Scholar
Rauschen, S, Schultheis, E, Pagel-Wieder, S, Schuphan, I, Eber, S (2009) Impact of Bt-corn MON88017 in comparison to three conventional lines on Trigonotylus caelestialium (Kirkaldy) (Heteroptera: Miridae) field densities. Trans. Res. 18: 203–214 Google ScholarPubMed
Rauschen, S, Schaarschmidt, F, Gathmann, A (2010) Occurrence and field densities of Coleoptera in the maize herb layer: Implications for Environmental Risk Assessment of genetically modified Bt-maize. Trans. Res. 19: 727–744 Google ScholarPubMed
Romeis, J, Meissle, M, Bigler, F (2006) Transgenic crops expressing Bacillus thuringiensis toxins and biological control. Nat. Biotechnol. 24: 63–71 Google ScholarPubMed
Romeis, J, Bartsch, D, Bigler, F, Candolfi, MP, Gielkens, MMC, Hartley, SE, Hellmich, RL, Huesing, JE, Jepson, PC, Layton, R, Quemada, H, Raybould, A, Rose, RI, Schiemann, J, Sears, MK, Shelton, AM, Sweet, J, Vaituzis, Z, Wolt, JD (2008) Assessment of risk of insect-resistant transgenic crops to nontarget arthropods. Nat. Biotechnol. 26: 203–208 Google ScholarPubMed
Sanvido, O, Widmer, F, Winzeler, M, Bigler, F (2005) A conceptual framework for the design of environmental post-market monitoring of genetically modified plants. Environ. Biosafety Res. 4: 13–27 Google ScholarPubMed
Sanvido, O, Romeis, J, Bigler, F (2009) An approach for post-market monitoring of potential environmental effects of Bt-maize expressing Cry1Ab on natural enemies. J. Appl. Entomol. 133: 236–248 Google Scholar
Schmitz, G, Bartsch, D (2001) Biozönotische Untersuchungen in Maisfeldern bei Bonn und Aachen. Mitt. Dtsch. Ges. Allg. Angew. Entomol. 13: 615–618 (in German) Google Scholar
Schnepf, E, Crickmore, N, van Rie, J, Lereclus, D, Baum, J, Feitelson, F, Zeigler, DR, Dean, DH (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. R. 62: 775–806 Google ScholarPubMed
Venables WN, Ripley BD (2002) Modern applied statistics with S. Fourth Edition. New York, Springer