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Detection of feral transgenic oilseed rapewith multiple-herbicide resistance in Japan

Published online by Cambridge University Press:  08 December 2006

Mitsuko Aono ,
Seiji Wakiyama ,
Masato Nagatsu ,
Nobuyoshi Nakajima ,
Masanori Tamaoki ,
Akihiro Kubo  and
Hikaru Saji
Mitsuko Aono
Affiliation:
Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Seiji Wakiyama
Affiliation:
Japan Wildlife Research Center, 3-10-10 Shitaya, Taito-Ku, Tokyo, 110-8676, Japan
Masato Nagatsu
Affiliation:
Japan Wildlife Research Center, 3-10-10 Shitaya, Taito-Ku, Tokyo, 110-8676, Japan
Nobuyoshi Nakajima
Affiliation:
Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Masanori Tamaoki
Affiliation:
Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Akihiro Kubo
Affiliation:
Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Hikaru Saji
Affiliation:
Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan

Abstract

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Repeated monitoring for escaped transgenic crop plants is sometimes necessary, especially in cases when the crop has not been approved for release into the environment. Transgenic oilseed rape (Brassica napus) was detected along roadsides in central Japan in a previous study. The goal of the current study was to monitor the distribution of transgenic oilseed rape and occurrence of hybridization of transgenic B. napus with feral populations of its closely related species (B. rapa and B. juncea) in the west of Japan in 2005. The progenies of 50 B. napus, 82 B. rapa and 283 B. juncea maternal plants from 95 sampling sites in seven port areas were screened for herbicide-resistance. Transgenic herbicide-resistant seeds were detected from 12 B. napus maternal plants growing at seven sampling sites in two port areas. A portion of the progeny from two transgenic B. napus plants had both glyphosate-resistance and glufosinate-resistance transgenes. Therefore, two types of transgenic B. napus plants are likely to have outcrossed with each other, since the double-herbicide-resistant transgenic strain of oilseed rape has not been developed intentionally for commercial purposes. As found in the previous study, no transgenic seeds were detected from B. rapa or B. juncea, and more extensive sampling is needed to determine whether introgression into these wild species has occurred.

Keywords

Brassicaestablishmentglufosinateglyphosateherbicideintrogressionoutcrossingtransgenic plant
Type
Research Article
Information
Environmental Biosafety Research , Volume 5 , Issue 2 , April 2006 , pp. 77 - 87
Copyright
© ISBR, EDP Sciences, 2006

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