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Preparation of Carboxymethyl Chitosan Microspheres and Their Application in Hemostasis

Published online by Cambridge University Press:  04 December 2015

Lu Liu ,
Qi Lv ,
Qingyun Zhang ,
Hui Zhu ,
Wei Liu ,
Guiru Deng ,
Yuqiang Wu ,
Chaofeng Shi ,
Hui Li  and
Lingzhi Li
Lu Liu*
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Qi Lv
Affiliation:
Institute of Disaster Medicine and Public Health, Affiliated Hospital of Chinese People’s Armed Police Force, Tianjin, China.
Qingyun Zhang
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Hui Zhu
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Wei Liu
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Guiru Deng
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Yuqiang Wu
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Chaofeng Shi
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Hui Li
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Lingzhi Li
Affiliation:
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin, China Institute of Disaster Medicine and Public Health, Affiliated Hospital of Chinese People’s Armed Police Force, Tianjin, China.
*
Correspondence and reprint requests to Dr Li Lingzhi, HuiZhiHuan Road 1, DongLi District, Tianjin, 300309, China (e-mail: [email protected]).
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Abstract

Objective

Chitosan (CS) is currently used as a hemostatic agent in emergencies and in military settings. However, its application is limited owing to its poor hydrophilia at neutral pH. Carboxymethyl chitosan (CMCS) is an important, water-soluble derivative of CS. In this study, we prepared CS and CMCS microspheres (CSMs and CMCSMs, respectively) and evaluated their hemostatic effect.

Methods

To prepare the microspheres of various sizes, we used the emulsion cross-linking technique. CMCSMs were also loaded with etamsylate (DIC). Clotting time in vitro and in a hepatic injury model was examined to evaluate the hemostatic effect.

Results

CMCSMs swelled more and clotted faster than did CSMs. CMCSMs loaded with DIC had no effect on hemostasis.

Conclusions

Both increasing material hydrophilicity and expanding the contact area promoted clotting, whereas chemical cross-linking hampered it because of decreased swelling. CMCSMs are promising candidates for the production of effective hemostatic agents. (Disaster Med Public Health Preparedness. 2017;11:660–667)

Keywords

emergency medicinemilitary medicineprotective deviceshemostatic agent in vitromicrosphere
Type
Original Research
Information
Disaster Medicine and Public Health Preparedness , Volume 11 , Issue 6 , December 2017 , pp. 660 - 667
Copyright
Copyright © Society for Disaster Medicine and Public Health, Inc. 2015 

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