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Maternal mental well-being during pregnancy and glucocorticoid receptor gene promoter methylation in the neonate

Published online by Cambridge University Press:  04 April 2016

Toby Mansell ,
Peter Vuillermin ,
Anne-Louise Ponsonby ,
Fiona Collier ,
Richard Saffery ,
Joanne Ryan  and
Barwon Infant Study Investigator Team
Toby Mansell
Affiliation:
Royal Children's Hospital University of Melbourne
Peter Vuillermin
Affiliation:
Royal Children's Hospital University of Melbourne Barwon Health Deakin University
Anne-Louise Ponsonby
Affiliation:
Royal Children's Hospital University of Melbourne
Fiona Collier
Affiliation:
Barwon Health Deakin University
Richard Saffery
Affiliation:
Royal Children's Hospital University of Melbourne
Joanne Ryan*
Affiliation:
Royal Children's Hospital University of Melbourne Hopital La Colombiere Universite Montpellier
*
Address correspondence and reprint requests to: Joanne Ryan, Murdoch Children's Research Institute, Royal Children's Hospital, Flemington Road, Parkville, Victoria 3052, Australia; E-mail: [email protected].
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Abstract

Maternal mental health during pregnancy has been linked to health outcomes in progeny. Mounting evidence implicates fetal “programming” in this process, possibly via epigenetic disruption. Maternal mental health has been associated with glucocorticoid receptor methylation (nuclear receptor subfamily 3, group C, member 1 [NR3C1]) in the neonate; however, most studies have been small (n < 100) and have failed to control for multiple testing in the statistical analysis. The Barwon Infant Study is a population-derived birth cohort with antenatal recruitment. Maternal depression and anxiety were assessed using the Edinburgh Postnatal Depression Scale and psychological distress using the Perceived Stress Scale. NR3C1 cord blood methylation levels were determined using Sequenom MassArray for 481 participants. Maternal psychological distress and anxiety were associated with a small increase in neonate NR3C1 methylation at specific CpG sites, thus replicating some previous findings. However, associations were only nominally significant and did not remain after correction for the number of CpG sites and exposures investigated. As the largest study to explore the relationship between maternal well-being and offspring NR3C1 cord blood methylation, our results highlight the need for caution when interpreting previous findings in this area. Future studies must ensure they are adequately powered to detect the likely small effect sizes while controlling for multiple testing.

Type
Regular Articles
Information
Development and Psychopathology , Volume 28 , Special Issue 4pt2: Epigenetics: Development, Psychopathology, Resilience, and Preventive Intervention , November 2016 , pp. 1421 - 1430
Copyright
Copyright © Cambridge University Press 2016 

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