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Maximal lactate steady state in swimming rats by a body density-related method of workload quantification

Published online by Cambridge University Press:  16 December 2011

Ivan Gustavo Masselli dos Reis ,
Gustavo Gomes de Araujo  and
Claudio Alexandre Gobatto
Ivan Gustavo Masselli dos Reis
Affiliation:
School of Physical Education, UNICAMP, Sao Paulo, Brazil
Gustavo Gomes de Araujo
Affiliation:
Federal University of Alagoas, Alagoas, Brazil
Claudio Alexandre Gobatto*
Affiliation:
School of Applied Sciences, UNICAMP, Sao Paulo, Brazil
*
*Corresponding author: [email protected]; [email protected]
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Abstract

The impact of body density (BD) on an animal's capacity to sustain a workload is important in the accuracy of evaluating swimming exercise in rats and the associated training protocols. The aim of this study was to quantify the influence of BD the maximal lactate steady-state (MLSS) workload in swimming rats. The BD of ten 90-day-old rats and sixteen 120-day-old rats was determined by underwater weighing, and their aerobic capacity was determined by the MLSS test. The MLSS blood concentration values were 4.11 ± 0.96 mmol l− 1 in the 90-day-old rats and 4.81 ± 1.49 mmol l− 1 in the 120-day-old rats. There was no significant (P>0.05) difference between these values. The older rats were more dense (P < 0.001) and showed a significantly increased (P < 0.001) absolute effort and relative-to-body-weight effort to keep themselves on the water surface when compared with the younger rats. BD can significantly affect an animal's capacity to sustain work within this age range, and individual fluctuation in effort should be determined to avoid mistakes when interpreting the results. This is particularly important in longitudinal studies in which the intervention or ageing process can modify the animal's body composition. Our results quantify the effects of BD on the performance of rats in the MLSS test.

Keywords

forced swimmingmaximal lactate steady-state
Type
Short Communication
Information
Comparative Exercise Physiology , Volume 7 , Issue 4 , November 2010 , pp. 179 - 184
Copyright
Copyright © Cambridge University Press 2011

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References

1Gobatto, CA, Mello, MAR, Sibuya, CY, Azevedo, JRM, Santos, LA and Kokubum, E (2001). Maximal lactate steady state in rats submitted to swimming exercise. Comparative Biochemistry and Physiology A 130: 21–27.CrossRefGoogle Scholar
2de Araujo, GG, Papoti, M, Manchado, FB, Mello, MAR and Gobatto, CA (2007). Protocols for hyperlactatemia induction in the lactate minimum test adapted to swimming rats. Comparative Biochemistry and Physiology: Part A 148: 888–892.CrossRefGoogle ScholarPubMed
3Gobatto, CA, Manchado-Gobatto, FB, Carneiro, LG, de Araujo, GG and dos Reis, IGM (2009). Maximal lactate steady state for aerobic evaluation of swimming mice. Comparative Exercise Physiology 6: 99–103.CrossRefGoogle Scholar
4Miller, HC and Darrow, DC (1941). Relation of serum and muscle electrolyte, particularly potassium, to voluntary exercise. American Journal of Physiology 132: 801–809.CrossRefGoogle Scholar
5Scheer, BT, Dorst, S, Codie, JF and Soule, DF (1947). Physical capacity of rats in relation to energy and fat content of the diet. American Journal of Physiology 149: 194–202.CrossRefGoogle ScholarPubMed
6Booth, FW, Laye, MJ and Spangenburg, EE (2009). Gold standards for scientists who are conducting animal-based exercise studies. Journal of Applied Physiology 108: 219–221.CrossRefGoogle ScholarPubMed
7Harri, M and Kuusela, P (1986). Is swimming exercise or cold exposure for rats? Acta Physiologica Scandinavica 126: 189–197.CrossRefGoogle ScholarPubMed
8McArdle, WD and Montoye, HJ (1966). The reliability of exhaustive swimming in the laboratory rat. Journal of Applied Physiology 21: 1431–1434.CrossRefGoogle ScholarPubMed
9Behnke, AR and Wilmore, JH (1974). Evaluation and Regulation of Body Building and Composition. Englewood Cliffs, NY: Prentice-Hall.Google Scholar
10Wilber, CG and Hunn, JB (1960). Swimming of albino mice. Journal of Applied Physiology 15: 704–705.CrossRefGoogle ScholarPubMed
11Wilber, CG (1959). Some factors which are correlated with swimming capacity in guinea pigs. Journal of Applied Physiology 14: 199–203.CrossRefGoogle ScholarPubMed
12Engel, PC and Jones, JB (1978). Causes and elimination of erratic blanks in enzymatic metabolic assays involving the use of NAD in alkaline hydrazine buffers: improved conditions for the assay of l-glutamate, l-lactate, and other metabolites. Analytical Biochemistry 88: 475–485.CrossRefGoogle Scholar
13Billat, VL, Sirvent, P, Py, G and Koralsztein, JP (2003). The concept of maximal lactate steady state: a bridge between biochemistry, physiology and sport science. Sports Medicine 33: 407–426.CrossRefGoogle Scholar
14Mader, A and Heck, H (1986). A theory of the metabolic origin of anaerobic threshold. International Journal of Sports and Medicine 7: 45–46.CrossRefGoogle ScholarPubMed
15Dawson, C and Horvath, SM (1970). Swimming in small laboratory animals. Medicine and Science in Sports and Exercise 2: 51–78.CrossRefGoogle ScholarPubMed