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Transformation of Adsorbed Aflatoxin B1 on Smectite at Elevated Temperatures

Published online by Cambridge University Press:  01 January 2024

Asma Sadia ,
Linda Dykes  and
Youjun Deng
Asma Sadia
Affiliation:
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
Linda Dykes
Affiliation:
Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77845, USA
Youjun Deng*
Affiliation:
Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77845, USA
*
*E-mail address of corresponding author: [email protected]
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Abstract

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Aflatoxins cause liver damage and suppress immunity. Through adsorption, smectites can be used to reduce the bioavailability of aflatoxins. To further reduce the toxicity of aflatoxins and to eliminate the treatments of aflatoxin-loaded smectites, the ability to degrade the aflatoxin adsorbed to non-toxic or less toxic compounds is desirable. The objective of the present study was to investigate the effects of temperature and the exchange cation on the transformation of adsorbed aflatoxin B1 on smectite. An AfB1-Ca-smectite (sm) complex was synthesized. To enhance the Lewis acidity of the complexes, the exchanged calcium in the complex was replaced with Mn and Cu to obtain AfB1-Mn-sm and AfB1-Cu-sm complexes, respectively. The aflatoxin-sm complexes and pure aflatoxin B1 were dried at 60°C in aluminum cups, and heated from 100 to 200°C in 25°C steps. Aflatoxin B1 and its transformation products were extracted with methanol after the heat treatment. The extracts were analyzed using UV spectroscopy, high performance liquid chromatography (HPLC)-fluorescence/UV, ultra-performance liquid chromatography (UPLC)-photodiode array (PDA), and electrospray ionization-tandem quadrupole-mass spectrometry (ESI-TQDMS). The solid residues were analyzed using Fourier-transform infrared spectroscopy (FTIR). The UV and FTIR spectra of the AfB1-sm clay residue extracts obtained after heating had decreased AfB1 peak intensities and shifted peak positions with increased heating temperature. Significant shifts in band positions and changes in the shape of the UV spectra were observed in the extracts from the AfB1-Ca-sm complex heated at 175°C, the AfB1-Cu-sm complex heated at 150°C, and the AfB1-Mn-sm complex heated at 125°C. The HPLC and UPLCMS analyses of AfB1-sm complex extracts indicated a gradual decrease in AfB1 concentration with increased heating temperature and the formation of aflatoxins B2, B2a, M1, M2, and other unidentified compounds. No new compound was observed in the extracts of pure aflatoxin B1 after a comparable heating experiment. These results suggest that smectite can effectively convert aflatoxin to other less toxic forms at elevated temperatures. Smectite ion exchange with Cu or Mn transition-metal cations and heat treatment induced more efficient conversion of the adsorbed aflatoxin B1 molecules to other compounds.

Keywords

AflatoxinHPLCSmectiteTransformationUPLC-MS
Type
Article
Information
Clays and Clay Minerals , Volume 64 , Issue 3 , June 2016 , pp. 220 - 229
Copyright
Copyright © Clay Minerals Society 2016

References

Cole, R.J. and Cox, R.H., 1981 Handbook of Toxic Metabolites New York Academic Press.Google Scholar
Deng, Y. Velázquez, A.L.B. Billes, F. and Dixon, J.B., 2010 Bonding mechanisms between aflatoxin B1 and smectite Applied Clay Science 50 92–98.CrossRefGoogle Scholar
Dhanasekaran, D. Shanmugapriya, S. Thajuddin, N. Panneerselvam, A., Ramon, GG-G, 2011 Aflatoxins and aflatoxicosis in humans and animals Aflatoxins — Biochemistry and Molecular Biology 221–254.CrossRefGoogle Scholar
Doyle, M.P. and Marth, E.H., 1978 Aflatoxin is degraded at different temperatures and pH values by mycelia of Aspergillus parasiticus European Journal of Applied Microbiology 6 95–100.CrossRefGoogle Scholar
Farag, R.S. Rashed, M.M. and Abo, H., 1996 Aflatoxin destruction by microwave heating International Journal of Food Sciences and Nutrition 41 197–208.CrossRefGoogle Scholar
Grant, P.G. and Phillips, T.D., 1998 Isothermal adsorption of aflatoxin B1 on HSCAS Clay Journal of Agricultural Food Chemistry 46 599–605.CrossRefGoogle ScholarPubMed
Herzallah, S. Alshawabkeh, K. and Al Fataftah, A., 2008 Aflatoxin decontamination of artificially contaminated feeds by sunlight, γ-radiation, and microwave heating Journal of applied Poultry Research 17 515–521.CrossRefGoogle Scholar
Hussain, A. Ali, J. and Akther, S., 2011 Degradation of aflatoxins by roasting in contaminated peanuts Pakistan Journal of Biochemistry and Molecular Biology 44 56–59.Google Scholar
Huwig, A. Freimund, S. Kappeli, O. and Dutler, H., 2001 Mycotoxin detoxification of animal feed by different adsorbents Toxicology Letters 122 179–188.CrossRefGoogle Scholar
International Agency for Research on Cancer (IARC) (2002) Monograph on the evaluation of the carcinogenic risk to humans: Some Traditional Herbal Medicines, some Mycotoxins, Naphthalene and Styrene, vol. 82. IARC, World Health Organization, France.Google Scholar
Liu, R. Jin, Q. Tao, G. Shan, L. Liu, Y. and Wang, X., 2010 LC-MS and UPLC-Quadrupole Time-of-Flight MS for identification of photodegradation products of aflatoxin B1 Chromatographia 71 107–112.CrossRefGoogle Scholar
Liu, R. Chang, M. Jin, Q. Huang, J. Liu, Y. and Wang, X., 2011 Degradation of aflatoxin B1 in aqueous medium through UV irradiation European Food Research and Technology 233 1007–1012.CrossRefGoogle Scholar
Mann, G.E. Codifer, L.P. and Dolear, F.G., 1967 Effect of heat on aflatoxin in oilseed meals Journal of Agricultural Food Sciences 15 1090–1092.Google Scholar
Méndez-Albores, A. Campos-Aguilar, A.Z. Moreno-Martínez, E. and Vázquez-Durán, A., 2013 Physical and chemical degradation of B-aflatoxins during the roasting and dutching of cocoa liquor Journal of Agricultural Science and Technology 15 557–567.Google Scholar
Perez-Flores, G.C. Moreno-Martínez, E. and Méndez-Albores, A.M., 2011 Effect of microwave heating during alkaline cooking of aflatoxin contaminated maize Journal of Food Science 76 49–52.CrossRefGoogle ScholarPubMed
Phillips, T.D. Clement, B.A. Kubena, L.F. and Harvey, R.B., 1990 Detection and detoxification of aflatoxins: prevention of aflatoxicosis and aflatoxin residues with hydrated sodium calcium aluminosilicate Veterinary and Human Toxicology 32 15–19.Google ScholarPubMed
Phillips, T.D. Clement, B.A. Park, D.L., D.L, E. and J.D, G., 1994 Approaches to reduction of aflatoxins in foods and feeds The Toxicology of Aflatoxins: Human Health, Veterinary, and Agricultural Significance San Diego, California, USA Academic Press 383–406.CrossRefGoogle Scholar
Phillips, T.D. Sarr, A.B. and Grant, P.G., 1995 Selective chemisorption and detoxification of aflatoxins by phyllosilicate clay Natural Toxins 3 204–213.CrossRefGoogle ScholarPubMed
Phillips, T.D. Afriyie-Gyawu, E. Williams, J. Huebner, H. Ankrah, N. Ofori-Adjei, D. Jolly, P. Johnson, N. Taylor, J. Marroquin-Cardona, A. Xu, L. Tang, L. and Wang, J.S., 2008 Reducing human exposure to aflatoxin through the use of clay: a review Food additives & contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 25, 134–45.Google ScholarPubMed
Piva, G.F.P. Galvano, F. Pietri, A. and Piva, A., 1995 Detoxification methods of aflatoxins A review. Nutrition Research 15 767–776.CrossRefGoogle Scholar
Pico, Y. and Barcelo, D., 2008 The expanding role of LC-MS in analyzing metabolites and degradation products of food contaminants Trends in Analytical Chemistry 27 821–835.CrossRefGoogle Scholar