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Modifying a Smectite using Organic Nutrients to Enhance its Efficacy at Removing Aflatoxin B1 from Corn Fermentation Solution

Published online by Cambridge University Press:  01 January 2024

Sabrina Sharmeen Alam  and
Youjun Deng
Sabrina Sharmeen Alam
Affiliation:
Department of Soil Science, University of Chittagong, Chattogram 4331, Bangladesh Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77845, USA
Youjun Deng*
Affiliation:
Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77845, USA
*
e-mail: [email protected]
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Abstract

Aflatoxins in contaminated corn do not degrade in corn fermentation solution (CFS) during biofuel production; rather, they are enriched in the co-product, dried distillers grain. Aflatoxin B1 (AfB1) is the most toxic form of all aflatoxins. Removing AfB1 from CFS is desirable to minimize its toxicity to animals. Smectites can adsorb AfB1 from aqueous solutions and, therefore, inactivate the toxin, but proteins in CFS inhibit the adsorption of AfB1 by smectites. The current study aimed to minimize the interference by CFS in adsorption of AfB1 on smectite by modifying a calcium-smectite (Ca-3MS) with a small nutritive organic compound, e.g. carnitine, choline, arginine, histidine, or tryptophan. The organo-smectites were characterized by X-ray diffraction (XRD) and Fourier-transform infrared (FTIR) spectroscopy, and adsorption of AfB1 in CFS by these composites was examined. Various degrees of intercalation of the organic nutrients into the smectites were observed with XRD and FTIR. After immersing the smectite and organo-smectites in the CFS, the d001 values of Ca-3MS expanded to ~1.82 nm due to protein interaction, but the organo-smectites were confined to ~1.39 nm, which indicated that the protein had limited access to the organo-smectite interlayers. The IR bands at ~1652, 1544, 1538, and 1454 cm–1 from the organo-smectites revealed, however, that complete protein inhibition was not achieved. The organo-smectites were capable of adsorbing AfB1 in simple aqueous solution with maximal adsorption capacity up to 0.55 mol kg–1. Significantly greater (p ≤ 0.05) AfB1 adsorption was achieved by choline- and carnitine-modified smectites compared with the original Ca-3MS in the presence of competing protein (pepsin) in simple aqueous solution. In real CFS, both AfB1 adsorption capacities (Qmax) and affinities (K) by all organo-smectites were greater (Qmax = up to 0.45 mol kg–1 and K = up to 0.165 μM–1) than those by Ca-3MS (Qmax = 0.22 mol kg–1 and K = 0.031 μM–1). The study suggested that using smectites modified with an organic nutritive compound could be an effective, economical, and safe strategy for removing mycotoxins, including aflatoxins, during biofuel production.

Keywords

AflatoxinsAmino acidsCarnitineCholineFermentationSmectite
Type
Original Paper
Information
Clays and Clay Minerals , Volume 70 , Issue 2: Special Issue: Biological toxins-clay interactions , April 2022 , pp. 196 - 208
Copyright
Copyright © The Author(s), under exclusive licence to The Clay Minerals Society 2022

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