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Tolerance of submergence in rice: gene studies using differential display technique

Published online by Cambridge University Press:  02 August 2007

Chen Yong-Hua ,
Zhao Sen ,
Yan Qin-Quan ,
Li Yang-Sheng ,
Wu Xing-Rong  and
Xiao Guo-Ying
Chen Yong-Hua
Affiliation:
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China Agronomy College of Hunan Agricultural University, Changsha 410128, China
Zhao Sen
Affiliation:
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
Yan Qin-Quan
Affiliation:
Agronomy College of Hunan Agricultural University, Changsha 410128, China
Li Yang-Sheng
Affiliation:
College of Life Sciences, Wuhan University, Wuhan 430072, China
Wu Xing-Rong
Affiliation:
Biochemistry Department, University of Missouri-Columbia, MO 65211, USA
Xiao Guo-Ying*
Affiliation:
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Gene expression profiles between submergence rice (Oryza sativa ssp. indica) varieties, tolerant FR13A and sensitive IR39595-503-2-1-2, under submergence stress were identified using differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR). A total of 1428 bands were amplified with 40 pairs of primers, of which 102 were significantly different between the two lines. The differential display ratio was 7.1%. Among 42 differential display bands derived from the submergence tolerant variety, the expression of seven fragments was confirmed by Northern blot analysis. The analysis of their sequences indicated that four of them showed high homology with genes related to a water stress response: genes encoding ATP-binding protein, isocitrate dehydrogenase, NADH dehydrogenase and terminal acetyltransferase, respectively. The remaining three fragments were novel cDNA fragments.

Keywords

mRNA differential displayOryza sativa ssp. indicasubmergence stress
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 2 , August 2007 , pp. 139 - 144
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(6): 894–898

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