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RNAi effects on regulation of endogenous acid invertase activity in potato (Solanum tuberosum L.) tubers

Published online by Cambridge University Press:  01 October 2008

Zhang Chi ,
Xie Cong-Hua ,
Song Bo-Tao ,
Liu Xun  and
Liu Jun
Zhang Chi
Affiliation:
National Vegetable Improvement Center (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
Xie Cong-Hua
Affiliation:
National Vegetable Improvement Center (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
Song Bo-Tao
Affiliation:
National Vegetable Improvement Center (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
Liu Xun
Affiliation:
National Vegetable Improvement Center (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
Liu Jun*
Affiliation:
National Vegetable Improvement Center (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

In plants, acid invertases are known to be the key enzymes cleaving sucrose into reducing sugars (RS) (glucose and fructose). To improve the quality of potato (Solanum tuberosum L.) chips, which is largely influenced by RS accumulation in tubers stored at low temperature, a part of acid invertase cDNA with hairpin RNA (hpRNA) structure was transformed into potato cv. N2. Detection of polymerase chain reaction (PCR) amplification and Northern blotting suggested that the RNA interference (RNAi) vector was successfully transformed into cv. N2. The analysis of acid invertase activity in the plantlets and microtubers of RNAi transgenic lines indicated that the expression of the acid invertase was significantly repressed by the activity of RNAi of plantlets by an average 69.8% (with the exception of line Ni-1) with a maximal decrease of 78% (line Ni-4), and the highest decrease of activity in microtubers of 68%. Compared with that of well-inhibited antisense inv transgenic plants, the comparative downregulation of RNAi suggests a distinct alteration of endogenous acid invertase activity and a potential strategy for post-transcriptional gene silencing (PTGS) in modulation of cold-sweetening in potato.

Keywords

potato (Solanum tuberosum L.)acid invertasecold-sweeteningRNA interference
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 107 - 112
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(1): 108–113

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