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RNA3 nucleotide sequence analyses of peanut stunt virus Mi strain

Published online by Cambridge University Press:  12 February 2007

Xu Ze-Yong ,
Yan Li-Ying ,
Chen Kun-Rong  and
Marcel Prins
Xu Ze-Yong*
Affiliation:
Key Laboratory of Genetic Improvement for Oil Crops, Ministry of Agriculture, Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Yan Li-Ying
Affiliation:
Key Laboratory of Genetic Improvement for Oil Crops, Ministry of Agriculture, Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Chen Kun-Rong
Affiliation:
Key Laboratory of Genetic Improvement for Oil Crops, Ministry of Agriculture, Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Marcel Prins
Affiliation:
Laboratory of Virology, Department of Plant Sciences, Wageningen University, Wageningen, The Netherlands
*
*Corresponding author: Email: [email protected]
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Abstract

Nucleotide sequence of full-length cDNA of peanut stunt virus (PSV) Mi strain RNA3 was determined and compared with those of PSV-ER and -J (subgroup I) and PSV-W (subgroup II), strains of cucumber mosaic virus (CMV) and tomato aspermy virus (TAV). PSV-Mi RNA3 consists of 2170 nt and has two open reading frames, encoding a putative movement protein (3a protein) and a coat protein (CP). PSV-Mi RNA3 is 77.7% and 78.5% identical to those of PSV-ER and -J, whereas it shares 76.6% identity with PSV-W. Nucleotide identity of 3a and cp genes between PSV strains Mi and ER, J and W was 78.3–79.3% and 74.4–77.8%, respectively. Amino acid identity of 3a and CP between PSV-Mi and -ER, -J and -W was 73.9–77.4% and 64.8–77.5%, respectively. RNA3 of PSV-Mi (GenBank accession no. AY775057) had a varied intercistronic and 5′-untranslated region compared with those of PSV strains ER, J and W. Results indicate that PSV-Mi represents a new PSV subgroup from China, designated as subgroup III.

Keywords

peanut stunt virussequence determinationsequence alignmentnew PSV subgroup
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 1 , April 2005 , pp. 33 - 38
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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