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Regeneration of sweet potato transgenic plants with oryzacystatin-I (OCI) gene

Published online by Cambridge University Press:  12 February 2007

Jiang Sheng-Jun ,
Liu Qing-Chang ,
Zhai Hong ,
Wu Li-Sha  and
Wang Yu-Ping
Jiang Sheng-Jun
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing 100094, China
Liu Qing-Chang*
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing 100094, China
Zhai Hong
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing 100094, China
Wu Li-Sha
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing 100094, China
Wang Yu-Ping
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing 100094, China
*
*Corresponding author: Email: [email protected]
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Abstract

Regeneration of sweet potato (Ipomoea batatas cv. Lizixiang) transgenic plants with oryzacystatin-I (OCI) gene was achieved using an Agrobacterium tumefaciens-mediated method. A. tumefaciens strain LBA4404, harbouring a binary vector pBinh with neomycin phosphotransferase II and OCI genes, was used. After 3 days of subculture, Lizixiang embryogenic suspension cultures were co-cultivated with LBA4404 (OD600nm=0.5) for 4 days. Next, the infected suspension cultures were first cultured for 5 days in MS medium with 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 300 mg/l carbencillin (Carb) but without kanamycin (Kan) and then transferred to MS medium supplemented with 2 mg/l 2,4-D, 50 mg/l Kan and 300 mg/l Carb for the selection culture. Four weeks after selection, 200 Kan-resistant cell aggregates (∼1 mm in size) from the embryogenic suspension cultures were transferred to MS solid medium supplemented with 2 mg/l 2,4-D, 50 mg/l Kan and 300 mg/l Carb, and eight embryogenic calluses were obtained. After transferring to MS medium supplemented with 1 mg/l ABA, 50 mg/l Kan and 100 mg/l Carb, these embryogenic calluses formed 13 plantlets via somatic embryogenesis. PCR and PCR–Southern blot analysis indicated that seven of the 13 plantlets were transgenic.

Keywords

Ipomoea batatastransgenic plantsoryzacystatin-I gene
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 2 , August 2004 , pp. 99 - 102
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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References

Atkinson, HJ, Urwin, PE, Hansen, E et al. (1998) Engineering resistance in the potato to potato cyst nematodes. In Marks, RJ and Brodie, BB (editors) Potato Cyst Nematodes, Biology, Distribution and Control. Wallingford, UK: CAB International, pp. 209–236.Google Scholar
Cipriani, G, Michaud, D, Brunelle, F et al. (1999) Expression of Soybean Proteinase Inhibitor in Sweet Potato. Impact on a Changing World. International Potato Center program Report.Lima:International Potato Center, pp. 271–277.Google Scholar
Gama, MCS and Leite, R et al. . Cordeiro AR et al. Jr (1996) Transgenic sweetpotato plants obtained by Agrobacterium tumefaciens -mediated transformation. Plant Cell Tissue and Organ Culture 46: 237–244.CrossRefGoogle Scholar
Gao, F, Gong, Y-F, Lin, Z-P et al. . (2001) Agrobacterium -mediated genetic transformation of Ipomoea batatas and regeneration of transgenic plants. Acta Agronomica Sinica 27: 751–756. (in Chinese with English abstract).Google Scholar
Kimura, T, Otani, M and Noda, T et al. . (2001) Absence of amylose in sweet potato ( Ipomoea batatas (L.) Lam.) following the introduction of granule-bound starch synthase I cDNA. Plant Cell Reports 20: 663–666.CrossRefGoogle Scholar
Liu, Q-C Lu, D-H, Ma B et al. . (1996) Cell suspension culture and efficient plant regeneration in sweetpotato, Ipomoea batatas (L.) Lam. Journal of Agricultural Biotechnology 4: 238–242.Google Scholar
Liu, Q-C, Zhai, H, Wang, Y-P et al. . (2001) Efficient plant regeneration from embryogenic suspension cultures of sweetpotato Vitro Cellular & Developmental Biology. Plant 37: 564–567.Google Scholar
Moran, R, Garcia, R and Lopez, A et al. . (1999) Transgenic sweet potato plants carrying the delta-endotoxin gene from Bacillus thuringinensis var tenebrionis. Plant Science 139: 175–184.CrossRefGoogle Scholar
Murata, T, Okada, Y, Saito, A et al. . (1997) Transformation by direct gene transfer in sweetpotato ( Ipomoea batatas (L.) Lam.) Proceedings of the International Workshop on Sweetpotato Production System Toward 21st Century 159–179.Google Scholar
Newell, CA, Lowe, JM, Merryweather, A (1995) Transformation of sweetpotato ( Ipomoea batatas (L.) Lam.) with Agrobacterium tumefaciens and regeneration of plants expressing cowpea trypsin inhibitor and snowdrop lectin. Plant Science 107: 215–227.CrossRefGoogle Scholar
Urwin, P, Lilley, C, McPherson, M et al. . (1997) Resistance to both cyst- and root-knot nematodes conferred by transgenic Arabidopsis expressing a modified plant cystatin. The Plant Journal 12: 455–461.CrossRefGoogle ScholarPubMed
Vain, P, Worland, B, Clarke, M et al. . (1998) Expression of an engineered cysteine proteinase inhibitor for nematode resistance in transgenic rice plant. Theoretical and Applied Genetics 96: 266–271.CrossRefGoogle Scholar