Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-23T12:20:15.685Z Has data issue: false hasContentIssue false
  • English
  • Français

Over-expression of GbRac1 gene in transgenic tobacco enhances disease resistance to Alternaria alternata in vitro

Published online by Cambridge University Press:  13 June 2008

Li Wei-Min ,
Wang Zhi-Xing  and
Jia Shi-Rong
Li Wei-Min
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Wang Zhi-Xing
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Jia Shi-Rong*
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
*Corresponding author. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

GbRac1 gene was cloned from Gossypium barbadense with degenerate primers and 3′-RACE. Northern blot analysis indicated that GbRac1 mRNA was expressed abundantly in G. barbadense seedlings inoculated with Verticillium dehliae compared with mock-inoculated plants. A plant constitutive expression vector pRac harbouring GbRac1 gene was constructed and leaf discs of tobacco (Nicotiana tabacum L. cv. NC89) were transformed with pRac by Agrobacterium-mediated transformation. Disease challenge test of detached leaves of the transgenic plants by inoculation with Alternaria alternata showed that resistance was enhanced dramatically compared with the non-transgenic plants. Results suggest that GbRac1 gene might have potential application in the genetic engineering of plants with enhanced disease resistance.

Keywords

Gossypium barbadenseGTP-binding protein Ractransgenic tobaccoAlternaria alternatadisease resistance
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 2 , August 2005 , pp. 73 - 77
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bokoch, GM (1994) Regulation of the human neutrophil NADPH oxidase by the Rac GTP-binding proteins. Current Opinion in Cell Biology 6: 212–218.CrossRefGoogle ScholarPubMed
Horsch, RB, Fry, JE, Hoffman, NL, Eichholts, D, Rogers, SG and Fraley, RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229–1231.CrossRefGoogle Scholar
Kawasaki, T, Henmi, K, Ono, E, Hatakeyama, S, Iwano, M, Satoh, H et al. , (1999) The small GTP-binding protein Rac is a regulator of cell death in plants. Proceedings of the National Academy of Sciences of the USA 96: 10922–10926.CrossRefGoogle ScholarPubMed
Ma, C, Jian, GL and Sun, WJ (1997) Present status, constraints and countermeasures in cotton breeding for Verticillium wilt resistance in China. Chinese Agricultural Science 30: 58–64.Google Scholar
Morel, JB and Dangl, JL (1997) The hypersensitive response and the induction of cell death in plants. Cell Death and Differentiation 4: 671–683.CrossRefGoogle ScholarPubMed
Ono, E, Wong, HL, Kawasaki, T, Hasegawa, M, Kodama, O and Shimamoto, K (2001) Essential role of the small GTPase Rac in disease resistance of rice. Proceedings of the National Academy of Sciences of the USA 98: 759–764.CrossRefGoogle ScholarPubMed
Park, J, Choi, HJ, Lee, S, Lee, T, Yang, Z and Lee, Y (2000) Rac-related GTP-binding protein in elicitor-induced reactive oxygen generation by suspension-cultured soybean cells. Plant Physiology 124: 725–732.CrossRefGoogle ScholarPubMed
Pennell, RI and Lamb, C (1997) Programmed cell death in plants. Plant Cell 9: 1157–1168.CrossRefGoogle ScholarPubMed
Potikha, TS, Collins, CC, Johnson, DI, Delmer, DP and Levine, A (1999) The involvement of hydrogen peroxide in the differentiation of secondary walls in cotton fibers. Plant Physiology 119: 849–858.CrossRefGoogle ScholarPubMed
Sambrook, J, Fritsch, EF and Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.Google Scholar
Vaux, DL and Korsmeyer, SJ (1999) Cell death in development. Cell 96: 245–254.CrossRefGoogle ScholarPubMed
Yang, ZB (2002) Small GTPase: versatile signaling switches in plants. Plant Cell 14(Suppl.): 375–388CrossRefGoogle ScholarPubMed