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Mutation breeding of Aspergillus niger strain LW-1 for high-yield β-mannanase production

Published online by Cambridge University Press:  01 October 2008

Zhang Shu-Fei ,
Song Jia-Huan ,
Wu Min-Chen ,
Sheng Jin-Ping  and
Li Jian-Fang
Zhang Shu-Fei
Affiliation:
School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
Song Jia-Huan
Affiliation:
School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
Wu Min-Chen*
Affiliation:
School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
Sheng Jin-Ping
Affiliation:
School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
Li Jian-Fang
Affiliation:
School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

A parent strain of Aspergillus niger LW-1 producing β-mannanase, preserved in our laboratory, was isolated. A strain, N-9, was screened out and further treated with vacuum microwave irradiation and ethyl methane sulphonate (EMS). A mutant strain, E-30, producing a high and stable yield of β-mannanase was obtained through screening by solid-state cultivation on the basic fermentation medium and several generations of bevel subculture. Its enzyme activity (36 675 U/g) was increased by 2.15 times compared to that of A. niger LW-1 (17 048 U/g). The production of high-yield β-mannanase by E-30 remained stable when maintained at 4°C for 2 months.

Keywords

β-mannanaseAspergillus nigervacuum microwave irradiationethyl methane sulphonate
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 153 - 158
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(2): 346–350

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