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Microfertilization of oocytes and culture of embryos in goat

Published online by Cambridge University Press:  12 February 2007

Zhao Xiao-E ,
Yang Yan-Fei ,
Liu Feng-Jun ,
Wang Yong-Sheng ,
Wu Hao ,
Li Yu-Qiang  and
Zhang Yong
Zhao Xiao-E
Affiliation:
Bio-engineering Institute, Northwest A&F University, Yangling 712100, China
Yang Yan-Fei
Affiliation:
Bio-engineering Institute, Northwest A&F University, Yangling 712100, China
Liu Feng-Jun
Affiliation:
Bio-engineering Institute, Northwest A&F University, Yangling 712100, China
Wang Yong-Sheng
Affiliation:
Bio-engineering Institute, Northwest A&F University, Yangling 712100, China
Wu Hao
Affiliation:
Bio-engineering Institute, Northwest A&F University, Yangling 712100, China
Li Yu-Qiang
Affiliation:
Bio-engineering Institute, Northwest A&F University, Yangling 712100, China
Zhang Yong*
Affiliation:
Bio-engineering Institute, Northwest A&F University, Yangling 712100, China
*
*Corresponding author: E-mail: [email protected]
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Abstract

In vitro development of goat embryos obtained by fertilizing oocytes using intracytoplasmic sperm injection (ICSI) was investigated. The results showed that the blastocyst rate was significantly higher (P<0.05) when the embryos were co-cultured with granular cells (GCs) in media CR1aa supplemented with 10% bovine follicular fluid (BFF) (22.2%) than with 5% (13.1%) and 15% BFF (2.7%). When embryos were co-cultured with GCs in SOFaa media, the highest blastocyst rate was obtained from supplementation with 10% BFF (25.1%), which was significantly different from 5% (12.9%) and 15% BFF (3.0%) groups. When 10% BFF and fetus bovine serum (FBS) were added into CR1aa or SOFaa media, the goat ICSI blastocyst rates were 22.6 and 26.9% or 5.8 and 6.1% respectively. These results suggest that both CR1aa and SOFaa could be used as culture media for goat ICSI embryos, 10% BFF could significantly increase the blastocyst rate and BFF was more efficient than FBS for the early in vitro development of goat ICSI embryos.

Keywords

blastocyst ratebovine follicular fluidfetus bovine serumgoatintracytoplasmic sperm injectionoocytessperm
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 1 , April 2006 , pp. 19 - 23
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

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