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Inheritance and resistance to insects in CryIA(c) transgenic cabbage

Published online by Cambridge University Press:  15 June 2007

Li Han-Xia ,
Yin Ruo-He ,
Lu Ya-Chun ,
Zhang Yu-Yang  and
Zhang Jun-Hong
Li Han-Xia*
Affiliation:
College of Horticulture and Forestry, Huazhong Agricultural University; National Vegetable Improvement Center, Wuhan 430070, China
Yin Ruo-He
Affiliation:
College of Horticulture and Forestry, Huazhong Agricultural University; National Vegetable Improvement Center, Wuhan 430070, China
Lu Ya-Chun
Affiliation:
College of Horticulture and Forestry, Huazhong Agricultural University; National Vegetable Improvement Center, Wuhan 430070, China
Zhang Yu-Yang
Affiliation:
College of Horticulture and Forestry, Huazhong Agricultural University; National Vegetable Improvement Center, Wuhan 430070, China
Zhang Jun-Hong
Affiliation:
College of Horticulture and Forestry, Huazhong Agricultural University; National Vegetable Improvement Center, Wuhan 430070, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Using hypocotyl segments of aseptic seedlings of cabbage (Brassica oleracea var. capitata) as explants, regenerated plants with kanamycin resistance were obtained mediated by Agrobacterium tumefaciens (strain LBA4404). The transformed plants with the CryIA(c) (Bt) gene were confirmed by Southern blotting analysis, indicating the integration of the transgene into the cabbage genome. The majority of the transgenic plants had only a single copy of the inserted CryIA(c) gene. Leaf section bioassays showed that resistance against larvae of diamondback moth in CryIA(c) transgenic cabbage was significantly enhanced. The inheritance patterns of the transgene in T1 offspring of transgenic cabbage were investigated using polymerase chain reaction (PCR) analysis and a kanamycin resistance test on the leaves of young seedlings. The results showed that dominant gene loci, CryIA(c) or neomycin phosphotransferase gene (NPTII), followed Mendelian inheritance, with a ratio of 3:1 segregation in T1 populations.

Keywords

cabbageBt transgenic plantsinsect resistance
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 1 , April 2007 , pp. 57 - 61
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(4): 546–550

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