Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-22T18:03:04.540Z Has data issue: false hasContentIssue false
  • English
  • Français

In vitro differentiation of chicken spermatogonial stem cells into adipocytes

Published online by Cambridge University Press:  03 March 2009

Yu Fei ,
Ge Jian-Hui ,
Ni Li-Gang ,
He Xian-Hong ,
Xu Qi  and
Li Bi-Chun
Yu Fei
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Ge Jian-Hui
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Ni Li-Gang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
He Xian-Hong
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Xu Qi
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Li Bi-Chun*
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China Institute of Animal Husbandry and Veterinary, Jiangsu Academy of Agricultural Science, Nanjing 210014, China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Spermatogonial stem cells (SSCs), which were isolated from chicken (Gallus gallus) embryo testes 16 days after laying, were cultured, subcultured, and induced into adipocytes in vitro. The differentiated cells were identified by oil red-O staining. Dexamethasone, insulin and 3-isobutyl-1-methylxanthine (IBMX) were tested for their induction potential. About 7–21 days after induction, SSCs differentiated into adipocytes, and the resulting adipocytes strongly expressed peroxisome proliferation activation receptor-γ (PPAR-γ). The assay outcome showed that an optimal treatment consisted of dexamethasone, insulin and IBMX application for 3 days and insulin for 1 day (3 cycles), then insulin for 21 days. The differentiation ratio was 85%, better than the combined use of dexamethasone, insulin and IBMX (P<0.01). However, the combination of the three derivatives triggered a stronger induction than any of them used alone (P<0.01). This study has demonstrated the potential of chicken embryonic SSCs to differentiate in vitro into adipocytes.

Keywords

chickenspermatogonial stem cellsadipocytesdifferentiation
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 3 , December 2008 , pp. 263 - 268
Copyright
Copyright © China Agricultural University 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(3): 450–455

References

Cao, J, Jin, Y and Zheng, CX (2002) Isolation and induced multi-differentiation of monkey marrow mesenchymal stem cells. Journal of The Fourth Military Medical University 23(24): 2305–2308 (in Chinese).Google Scholar
Chen, JJ and London, IM (1981) Hemin enhances the differentiation of mouse 3T3 cells to adipocytes. Cell 26(1): 117–122.CrossRefGoogle ScholarPubMed
El Hadri, K, Pairault, J and Feve, B (1996) Triiodothyronine regulates beta 3-adrenoceptor expression in 3T3-F442A differentiating adipocytes. European Journal of Biochemistry 239(2): 519.CrossRefGoogle ScholarPubMed
Gagnon, A, Dods, P and Roustan-Delatour, N (2001) Phosphatidylinositol-3,4,5-trisphosphate is required for insulin-like growth factor 1-mediated survival of 3T3-L1 preadipocytes. Endocrinology 142: 205–212.CrossRefGoogle ScholarPubMed
Gregoire, FM, Smas, CM and Sul, HS (1998) Understanding adipocyte differentiation. Physiological Review 78(3): 783–790.CrossRefGoogle ScholarPubMed
Jessen, BA and Tevens, GJ (2002) Expression profiling during adipocyte differentiation of 3T3-L1 fibroblasts. Gene 299(1): 95–102.CrossRefGoogle ScholarPubMed
Klemm, DJ, Roesler, WJ, Boras, T, Colton, LA, Felder, K and Reusch, JE-B (1998) Insulin stimulates cAMP-response element binding protein activity in HepG2 and 3T3-L1 cell line. Journal of Biological Chemistry 273: 917–923.CrossRefGoogle Scholar
Li, SF, Huang, DQ, Lu, XF, et al. (2003) Study on rabbit mesenchymal stem cells differentiation to the adipogenic or osteogenic lineage in vitro. Journal of Biomedical Engineering 20(2): 209–213 (in Chinese).Google ScholarPubMed
Lim, JY, Jeun, SS, Lee, KJ, et al. (2006) Multiple stem cell traits of expanded rat bone marrow stromal cells. Experimental Neurology 199(2): 416–426.Google Scholar
Naruse, K, Urabe, K, Mukaida, T, et al. (2004) Spontaneous differentiation of mesenchymal stem cells obtained from fetal rat circulation. Bone 35(4): 850–858.CrossRefGoogle ScholarPubMed
Oatley, JM and Brinster, RL (2006) Spermatogonial stem cells. Methods in Enzymology 419: 259–282.CrossRefGoogle ScholarPubMed
Saladin, R, Fajas, L, Dana, S, Halvorsen, Y-D, Auwerx, J and Briggs, M (1999) Differential regulation of peroxisome proliferator activated receptor gamma 1 (PPAR gamma 1) and PPAR gamma 2 messenger RNA expression in the early stages of adipogenesis. Cell Growth and Differentiation 10: 43–48.Google Scholar
Schlatt, S (2002) Spermatogonial stem cell preservation and transplantation. Molecular Cell Endocrinology 187(122): 107–111.CrossRefGoogle ScholarPubMed
Tang, QQ, Jiang, MS and Lane, MD (1999) Repressive effect of Sp1 on the C/EBPalpha gene promoter: Role in adipocyte differentiation. Molecular Cell Biology 19: 4855–4865.CrossRefGoogle Scholar
Wu, H, Li, BC, Zhou, GY, Sun, SY and Qin, J (2007) Study on the separation methods and culture of chicken testicular cells. Chinese Journal of Veterinary Medicine 43(3): 11–16.Google Scholar
Yamaguchi, T, Chattopadhyay, N and Kifor, O (1998) Extracellular calcium (Ca2+(o))-sensing receptor in a murine bone marrow-derived stromal cell line (ST2): potential mediator of the action of Ca2+(o) on the function of ST2 lines. Endocrinology 139: 3561–3568.CrossRefGoogle Scholar