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Genetic diversity analysis of Mystus guttatus from Xijiang Branch of Pearl River
Published online by Cambridge University Press: 02 August 2007
Abstract
The spotted longbarbel catfish (Mystus guttatus) is a member of the family Bagridae and is mainly distributed in the Pearl River Valley and Nandujiang River of Hainan Island. It is one of the four most famous fishes in the history of Pearl River Valley. Nowadays, M. guttatus has become a potential target for aquaculture in China due to its high nutritional value, but its genetic background is still unknown. This study was carried out to estimate its population genetic diversity in Pearl River. The random amplified polymorphic DNA (RAPD) technique was performed on 30 samples, using 20 random primers of length 10 nucleotides. The results showed that each primer amplified 3–9 bands per sample. Twenty primers produced 3210 well-amplified DNA fragments with reproducible band patterns and the average number of amplified DNA fragments reached 107 in each individual. Broadly speaking, 48 (44.9%) of 107 loci were polymorphic. Only the S30 primer did not produce polymorphic loci. The genetic distance of individuals ranged from 0.0467 to 0.2804. The average genetic distance among the 30 individuals was 0.1526±0.037. Genetic data in this study could be useful in genetic resource assessment as well as in wild population conservation and exploitation of M. guttatus in the future.
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- Research Article
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- Copyright © China Agricultural University and Cambridge University Press 2007
Footnotes
First published in Journal of Agricultural Biotechnology 2007, 15(1): 58–62
References
Apostol, BL (1993) Estimation of the number of full sibling families at an oviposition site using RAPD-PCR markers: applications to the mosquito Aedes aegypti. Theoretical and Applied Genetics 86: 991–1000.CrossRefGoogle Scholar
Li, MY, Zhang, HQ, Zhu, JJ, et al. (2004) Genetic variation between cultured population from Zhejiang Province and natural population from Burma of Macrobrachium rosenbergii revealed by RAPD. Journal of Fisheries of China 28(4): 360–364 (in Chinese with English abstract).Google Scholar
Liu, ZZ, Cai, WQ and Li, SF (2004) Analysis of genetic variations of five populations in Trionyx sinensis by RAPD. Journal of Fisheries of China 28(2): 119–125 (in Chinese with English abstract).Google Scholar
Lu, KX (1990) The fisheries resource in Pearl River. Guangzhou: Science and Technology Press of Guangdong, pp. 84–121 (in Chinese).Google Scholar
Nei, M and Li, WH (1985) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America 76: 5269–5273.CrossRefGoogle Scholar
Pearl River Fisheries Research Institute, Chinese Academy Fishery of Sciences (1991) Freshwater fish records of Guangdong. Guangzhou: Science and Technology Press of Guangdong, pp. 314–316 (in Chinese).Google Scholar
Shen, X, Zhou, KY and Wang, YQ (1999) RAPD analysis of Pit-vipers of the genus Agkistrodon in China. Acta Zoologica Sinica 45(1): 40–48 (in Chinese with English abstract).Google Scholar
Su, TF, Cai, YC, Zhang, DC and Jiang, SG (2002) RAPD analysis of three cultured populations of Pinctada martensii. Journal of Fishery Sciences of China 9(2): 106–109 (in Chinese with English abstract).Google Scholar
Wang, JW, Wang, W and Cui, YS (2000) Genetic diversity of three Gobiocypris rarus populations and one in breeding stock. Chinese Biodiversity 8(3): 241–247 (in Chinese with English abstract).Google Scholar
Xiao, TY, Zhang, XW, Zhang, HY, Tang, XB, Su, JM and Liu, Z (2004) RAPD analysis of genomic DNA among 4 species of Pelteobargrus of Dongting Lake. China Biotechnology 24(3): 84–89 (in Chinese with English abstract).Google Scholar
Yi, L, Wang, W, Li, MY and Liu, HZ (2001) A study of the geographical differentiation of Rhodeus ocellatus based on RAPD analysis. Acta Hydrobiologica Sinica 25(3): 301–304 (in Chinese with English abstract).Google Scholar
Zhang, DC and Yu, LN (1999) Studies on genetic diversity of bighead carp (Aristichthys nobilis) in the Yangtze River. Journal of Wuhan University (Nature Science Edition) 45(6): 857–860 (in Chinese with English abstract).Google Scholar
Zhang, SM, Deng, H, Wang, DQ and Yu, LN (2001) Population structure and genetic diversity of Silver Carp and Grass Carp from populations of Yangze River. Acta Hydrobiologica Sinica 25(4): 324–330 (in Chinese with English abstract).Google Scholar
Zhao, WX, Sun, YH, Shao, XL, et al. (2001) Study on genetic diversity of Silurus asotus (Linnaeus) and Ictalurus nebulosus with RAPD technique. Journal of Wuhan University (Nature Science Edition) 47(6): 782–784 (in Chinese with English abstract).Google Scholar
Zhou, L, Fan, LC and Gui, JF (1998) RAPD analysis of incorporation of heterologous genetic materials in multiple species of silver crucian carp. Acta Hydrobiologica Sinica 22(4): 301–306 (in Chinese with English abstract).Google Scholar
Zhu, XP, Chen, KC, Xie, G, et al. (2005a) The artificial propagation and embryonic development of spotted longbarbel catfish Mystus guttatus in ponds. Journal of Dalian Fisheries University 20(4): 352–354 (in Chinese with English abstract).Google Scholar
Zhu, XP, Du, HJ, Zhou, L, Li, MY and Gui, JF (2005b) Genetic diversity analysis of Chinese three-keeled pond turtle (Chinemys reevesii Gray) by RAPD. Acta Hydrobiologica Sinica 29(2): 42–46 (in Chinese with English abstract).Google Scholar