Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-22T22:57:32.311Z Has data issue: false hasContentIssue false
  • English
  • Français

Expression of modified VHb gene and fused insectidal gene in tobacco

Published online by Cambridge University Press:  27 June 2008

Wang You-Ru ,
Cui Hong-Zhi  and
Guo San-Dui
Wang You-Ru*
Affiliation:
Hubei Normal University, Huangshi 435002, China Biotechnology Research Institute of Chinese Academy of Agricultural Science, Beijing 100081, China
Cui Hong-Zhi
Affiliation:
Biotechnology Research Institute of Chinese Academy of Agricultural Science, Beijing 100081, China
Guo San-Dui
Affiliation:
Biotechnology Research Institute of Chinese Academy of Agricultural Science, Beijing 100081, China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The highly efficient plant binary expression vector pGBIF4ABCVHB was constructed using the artificially modified, synthesized VHb gene and fused insectidal gene (GFMcryIA and CPTI). Transgenic tobacco plants were obtained by Agrobacterium-mediated transformation. For 32 plants, the bivalent genes were inserted successfully into the tobacco genome and detected by polymerase chain reaction (PCR) amplification. Southern blot and Western blot analyses showed that VHb gene was expressed in the transgenic plants. Toxicity assay indicated that the fused insectidal gene expressed pesticidal toxin protein. The net weight of transgenic tobacco plants exceeded that of non-transgenic ones by 8%. Compared to non-transgenic tobacco plants, transgenic plants appeared to be high-yielding, insect-resistant varieties.

Keywords

VHb genefused insectidal genetransgenic plantpest resistanceyield
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 27 - 31
Copyright
Copyright © China Agricultural University 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(6): 982–986

References

Cui, HZ and Guo, SD (1998a) Insect resistant transgenic plant transformed with Bt gene. Journal of Agricultural Biotechnology 6(2): 166–172.Google Scholar
Cui, HZ and Guo, SD (1998b) The construction of expression vector harbouring bivalent pesticidal genes and their expression in tobacco. Journal of Agricultural Biotechnology 1(2): 7–13.Google Scholar
Dikshit, KL, Dikshit, RP and Webster, DA (1990) Study of Vitreoscilla globin (vgb) gene expression and promoter activity in E. coli through transcriptional fusion. Nucleic Acids Research 18(14): 4149–4155.CrossRefGoogle Scholar
Guo, SD, Cui, HZ and Xia, LQ (1999) Development of bivalent insect resistant transgenic cotton plants. Scientia Agricultura Sinica 32(3): 1–7.Google Scholar
Jelenkovic, G, Billngs, S and Chen, Q (1998) Transformation of eggplant with synthetic cryIIIA gene produces a high level of resistance to the Colorado potato bettle. American Society of Horticultural Science 123(1): 19–25.CrossRefGoogle Scholar
Li, HX, Yin, RH, Lu, YC and Zhang, JH (2006) Inheritance and resistance to insect in CryIA(c) transgenic cabbage. Journal of Agricultural Biotechnology 14(4): 546–550.Google Scholar
Ni, WC, Zhang, ZL and Guo, SD (1998) The cultivation of transgenic cotton resistant to pest. Scientia Agricultura Sinica 31(2): 8–13.Google Scholar
Sambrook, J and Russell, DW (2002) Molecular Cloning: A Laboratory Manual, 3rd ed. Beijing: Scientific Press, pp. 597–701.Google Scholar
Wang, GY, Du, TB and Zhang, H (1998) Transfer of Bt-toxin protein gene into maize by particle bombardment and regeneration of transgenic plants. Science in China, Ser B 38(9): 817–824.Google Scholar
Wang, ZB and Guo, SD (1999) Expression of two insect-resistant genes cryIA(bc)/gna in transgenic tobacco plants results in added protection against both cotton boll worm and aphids. Chinese Science Bulletin 44(19): 2068–2074.Google Scholar
Wu, DL, Cui, HZ and Guo, SD (2001) Construction of plant expression vector harboring a fused insecticidal gene and development of transgenic tobacco plants. Scientia Agricultura Sinica 34(5): 465–468.Google Scholar
Wu, QW, Cui, HZ and Guo, SD (2004a) The synthesis of the vgbM gene with plant preferential codon and its function identification in E. coli. Scientia Agricultura Sinica 37(10): 1439–1443.Google Scholar
Wu, XM, Chen, DH and Miao, Y (2004b) Studies on TI gene transformation to the tobacco plants. Journal of Xiamen University (Natural Science) 43(5): 706–709.Google Scholar
Yan, XP (2003) Transgenic Plant. Beijing: Scientific Press, pp. 1–240.Google Scholar
Zhao, FY, Xie, LX and Tian, YC (2005) Glyphosate resistant and boll worm resistant transgenic cotton plants with the aroAM12 and Bts1m genes. Acta Agronomica Sinica 31(1): 108–113.Google Scholar