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Effects of donor sex and different cell treatments on development of yak–bovine interspecies nuclear transfer embryos

Published online by Cambridge University Press:  27 June 2008

Liu Ying ,
Zhu Shi-En ,
Li Rong ,
Wang Li-Li ,
Wang Hai-Ping ,
Ding Fang-Rong ,
Li Jing ,
Li Song  and
Dai Yun-Ping
Liu Ying
Affiliation:
Animal Sci-tech College, China Agricultural University, Beijing 100094, China Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Zhu Shi-En
Affiliation:
Animal Sci-tech College, China Agricultural University, Beijing 100094, China
Li Rong
Affiliation:
Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Wang Li-Li
Affiliation:
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100094, China
Wang Hai-Ping
Affiliation:
Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Ding Fang-Rong
Affiliation:
Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Li Jing
Affiliation:
Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Li Song
Affiliation:
Geneprint Biotechnology Co. Ltd, Beijing 100094, China
Dai Yun-Ping*
Affiliation:
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

The purpose of this study was to evaluate the effects of donor sex, treatments of cell cycle synchronization and donor nuclei obtained from fresh or frozen–thawed conditions on developmental competence of yak–bovine interspecies nuclear transfer embryos. Bovine (Bos taurus) oocytes were used as recipients and yak (Bos grunniens) ear fibroblast cells were used as donors. Results indicated that the development rate of male blastocysts was higher than that of female (56.6% versus 39.5%, P<0.05), whereas cleavage and total cell number showed no difference between the two groups. No significant difference was observed in the development and quality of blastocysts with donor cells treated by serum starvation or contact inhibition, and there was no significant difference in embryo development with fresh or frozen–thawed donor cells, whereas the cleavage rate in the group of frozen–thawed cells was significantly lower than that of the fresh cell group (54.5% versus 78.2%, P<0.05). The results demonstrated that donor sex could impact the developmental competence of yak–bovine interspecies nuclear transfer embryos, whereas different treatments of cell cycle synchronization and freezing had little influence.

Keywords

yakinterspecies nuclear transferdonor cellembryo development
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 55 - 60
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(1): 61–65

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