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Diversity of endophytic diazotrophs isolated from Bambusa blumeana in Guangdong province

Published online by Cambridge University Press:  02 August 2007

How Wei ,
Peng Gui-Xiang ,
Xu Zhi-Jun ,
Chen Shi-Xian  and
Tan Zhi-Yuan
How Wei
Affiliation:
Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
Peng Gui-Xiang
Affiliation:
College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China
Xu Zhi-Jun
Affiliation:
Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
Chen Shi-Xian
Affiliation:
Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
Tan Zhi-Yuan*
Affiliation:
Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Endophytic bacteria isolated from Guangdong bamboo (Bambusa blumeana) plants were obtained under aerobic and anaerobic cultural conditions. Using the acetylene reduction assay (ARA), 40 strains were identified as diazotrophs. Diazotrophic isolates were grouped into five clusters (I, II, III, IV and V) by SDS-PAGE whole-cell protein patterns. The analysis of full-length 16S rDNA sequences of the representative strains of each group indicated that the endophytic diazotrophs showed great diversity and belonged to the genera Azospirillum (α-subclass of proteobacteria), Escherichia and Pseudomonas (γ-subclass of proteobacteria), and Aquaspirillum (β-subclass of proteobacteria).

Keywords

endophytic diazotrophsBambusa blumeanamicrobial diversity
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 2 , August 2007 , pp. 105 - 109
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(2): 290–294

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