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Development of an EST-SSR marker in Panax ginseng

Published online by Cambridge University Press:  01 October 2008

Yang Cheng-Jun ,
Wang Jun ,
Mu Li-Qiang ,
Li Shao-Chen ,
Liu Guan-Jun  and
Hu Chang-Qun
Yang Cheng-Jun
Affiliation:
College of Forestry, Northeast Forestry University, Harbin 150040, China
Wang Jun*
Affiliation:
College of Forestry, Northeast Forestry University, Harbin 150040, China
Mu Li-Qiang
Affiliation:
College of Forestry, Northeast Forestry University, Harbin 150040, China
Li Shao-Chen
Affiliation:
College of Forestry, Northeast Forestry University, Harbin 150040, China
Liu Guan-Jun
Affiliation:
College of Forestry, Northeast Forestry University, Harbin 150040, China
Hu Chang-Qun
Affiliation:
Jilin Provincial Academy of Forestry Science, Changchun 130033, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

A total of 791 microsatellites (SSRs) were isolated from 7055 Panax ginseng expressed sequence tags (ESTs). According to primer design criteria, 68 primer pairs for EST-SSR were designed. Under an appropriate polymerase chain reaction (PCR) system, all EST-SSR primer pairs were screened against genomic DNA of Ji'anchangbo and Fusong'ermaya from Panax ginseng, and 43 EST-SSR primer pairs out of the above 68 resulted in PCR products. Then, all 43 pairs were detected in nine P. ginseng, two Panax quinquefolius and two Acanthopanax senticosus cultivars for polymorphisms, and 26 pairs (60.47%) were found to be polymorphic, accounting for 38.23% of the total number of designed primer pairs. These results demonstrate the possibility of developing EST-SSR markers using P. ginseng ESTs.

Keywords

Panax ginsengEST-SSRmarker
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 175 - 181
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(1): 114–120

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