Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-23T20:33:52.355Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of a quorum sensing signal molecule of Acidovorax avenae subsp. citrulli and its regulation of pathogenicity

Published online by Cambridge University Press:  24 April 2009

Chen Tao ,
Qian Guo-Liang ,
Yang Xiao-Li ,
Ma Jun-Yi ,
Hu Bai-Shi  and
Liu Feng-Quan
Chen Tao
Affiliation:
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, China
Qian Guo-Liang
Affiliation:
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, China
Yang Xiao-Li
Affiliation:
Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Ma Jun-Yi
Affiliation:
Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Hu Bai-Shi*
Affiliation:
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, China
Liu Feng-Quan
Affiliation:
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

An efficient AHL (N-acyl-homoserine lactone) bioassay strain, JZA1, of Agrobacterium tumefaciens was used to detect the AHL production from Acidovorax avenae subsp. citrulli [the pathogen causing bacterial fruit blotch (BFB) of melons], and the results showed that A. avenae subsp. citrulli produced a 3-O-C8-homoserine (HSL) type signal molecule. Gene aiiA, which could degrade AHL molecules, was transformed into A. avenae subsp. citrulli strain NJF10, creating strain NJF10-aiiA. The AHL production from NJF10-aiiA was significantly reduced compared with wild-type NJF10. Inoculation tests showed that NJF10-aiiA had an obvious reduction of virulence on watermelon fruits. Our finds showed that AHL production by A. avenae subsp. citrulli was related to its pathogenicity. This work might provide a novel way to control BFB by QS (quorum sensing) interference.

Keywords

bacterial fruit blotch of melonsquorum sensingaiiA genesignal interference
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 1 , April 2009 , pp. 49 - 53
Copyright
Copyright © China Agricultural University 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Dong, YH, Xu, JL, Li, XZ and Zhang, LH (2000) AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proceedings of the National Academy of Sciences of the USA 97(7): 3526–3531.CrossRefGoogle ScholarPubMed
Dong, YH, Wang, LH, Xu, JL, Zhang, HB, Zhang, XF and Zhang, LH (2001) Quenching quorum sensing-dependent bacterial infection by an N-acyl homoserine lactonase. Nature 411: 813–817.CrossRefGoogle ScholarPubMed
Dong, YH, Zhang, XF, Xu, JL and Zhang, LH (2004) Insecticidal Bacillus thuringiensis silences Erwinia carotovora virulence by a new form of microbial antagonism – signal interference. Applied and Environmental Microbiology 70: 954–960.CrossRefGoogle ScholarPubMed
Fuqua, WC and Winans, SC (1994) A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. Journal of Bacteriology 176: 2796–2806.CrossRefGoogle ScholarPubMed
Fuqua, WC, Winans, SC and Greenberg, EP (1994) Quorum sensing in bacteria: The LuxR-LuxI family of cell density-responsive transcriptional regulators. Journal of Bacteriology 176: 269–275.CrossRefGoogle ScholarPubMed
Miller, MB and Bassler, BL (2001) Quorum sensing in bacteria. Annual Review of Microbiology 55: 165–199.CrossRefGoogle ScholarPubMed
O'Brien, RG and Martin, HL (1999) Bacterial blotch of melons caused by strains of Acidovorax avenae subsp. citrulli. Australian Journal of Experimental Agriculture 39(4): 479–485.CrossRefGoogle Scholar
Reimmann, C, Ginet, N, Michel, L, et al. (2002) Genetically programmed autoinducer destruction reduces virulence gene expression and swarming motility in Pseudomonas aeruginosa PAO1. Microbiology 148: 923–932.CrossRefGoogle ScholarPubMed
Sambrook, J and Russell, DW (1989) Molecular Cloning: A Laboratory Manual, 3rd ed.Cold Spring Harbor: Cold Spring Harbor Laboratory Press.Google Scholar
Shaw, PD, Ping, G, Daly, SL, et al. (1997) Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography. Proceedings of the National Academy of Sciences of the USA 94: 6036–6041.CrossRefGoogle ScholarPubMed
Somodi, GC, Jones, JB, Hopkins, DL, et al. (1991) Occurrence of a bacterial watermelon fruit blotch in Florida. Plant Disease 75(10): 1053–1056.CrossRefGoogle Scholar
Zhao, TC, Sun, FZ, Wang, BW and Hui, WG (2001) Pathogen identification of Hami melon bacterial fruit blotch. Acta Phytopathologica Sinica 31(4): 357–364 (in Chinese with English abstract).Google Scholar
Zhu, J, Chai, YR, Zhong, ZT, Li, SP and Winans, SC (2003) Agrobacterium bioassay strain for ultrasensitive detection of N-acylhomoserine lactone-type quorum-sensing molecules: detection of autoinducers in Mesorhizobium huakuii. Applied and Environmental Microbiology 69: 6949–6953.CrossRefGoogle ScholarPubMed