Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-05T06:57:01.911Z Has data issue: false hasContentIssue false
  • English
  • Français

CRS-PCR polymorphisms of the GHR gene and its relationship with milk production traits in Chinese Holstein cows

Published online by Cambridge University Press:  29 January 2010

Wang Li-Juan ,
Li Qiu-Ling ,
Wang Chang-Fa ,
Wang Hong-Mei ,
Li Jian-Bin ,
Gao Yun-Dong ,
Hou Ming-Hai  and
Zhong Ji-Feng
Wang Li-Juan
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China College of Public Health, Shandong University, Jinan 250012, China
Li Qiu-Ling
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Wang Chang-Fa
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Wang Hong-Mei
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Li Jian-Bin
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Gao Yun-Dong
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Hou Ming-Hai
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
Zhong Ji-Feng*
Affiliation:
Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan 250100, China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Single-nucleotide polymorphisms (SNPs) of exon 8 of the GHR gene were detected in Chinese Holstein cows by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The results showed that exon 8 of the GHR gene digested by TasI could be divided into two kinds of alleles and three kinds of genotypes. The frequencies of allele A and T were 0.6339 and 0.3661, respectively. The frequencies of genotypes AA, AT and TT were 0.459, 0.350 and 0.191, respectively. Sequencing showed one single nucleotide mutation T→A at 4962 bp of the gene in genotype TT when compared with genotype AA, and this mutation resulted in an amino acid change of phenylalanine (TTT)→tyrosine (TAT). The result of χ2 testing indicated that the genotypic frequency of the GHR gene digested by TasI did not fit with Hardy–Weinberg equilibrium in this population (P<0.05). The least-square mean of the fat percentage of this population was higher for genotype TT than for genotype AA (P<0.05).

Keywords

Chinese Holstein cattleGHR genemilk production traitsSNPsCRS-PCR
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 3 , December 2009 , pp. 215 - 219
Copyright
Copyright © China Agricultural University 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aggrey, SE, Yao, J, Sabour, MP, et al. (1999) Markers within the regulatory region of the growth hormone receptor gene and their association with milk-related traits in Holsteins. Heredity 90: 148–151.Google Scholar
Blott, S, Kim, J, Moisio, S, et al. (2003) Molecular dissection of a quantitative trait locus: a phenylalanine-to-tyrosine substitution in the transmembrane domain of the bovine growth hormone receptor is associated with a major effect on milk yield and composition. Genetics 163: 253–266.CrossRefGoogle Scholar
Curi, RA, Oliveira, HN, Silveira, AC and Lopes, CR (2000) Effects of polymorphic microsatellites in the regulatory region of IGF1 and GHR on growth and carcass traits in beef cattle. Animal Genetics 36: 58–62.CrossRefGoogle Scholar
Di Stasio, L, Destefanis, G, Brugiapaglia, A, Albera, A and Rolando, A (2005) Polymorphism of the GHR gene in cattle and relationships with meat production and quality. Animal Genetics 36: 138–140.CrossRefGoogle ScholarPubMed
Ge, W, Davis, ME, Hines, HC and Irvin, KM (2000) Rapid communication: single nucleotide polymorphisms detected in exon 10 of the bovine growth hormone receptor gene. Animal Science 78: 2229–2230.CrossRefGoogle ScholarPubMed
Parmentier, I, Portetelle, D, Gengler, N, et al. (1999) Candidate gene markers associated with somatotropic axis and milk selection. Domestic Animal Endocrinology 17: 139–148.CrossRefGoogle ScholarPubMed
Qin, QM, Xu, SZ and Gao, X (2007) Analysis of the correlations between growth hormone receptor gene polymorphism and body size indexes in cattle. Hereditas 29: 190–194.Google ScholarPubMed
Viitala, S, Szyda, J, Blott, S, et al. (2006) The role of the bovine growth hormone receptor and prolactin receptor genes in milk, fat and protein production in Finnish Ayrshire dairy cattle. Genetics 173: 2151–2164.Google Scholar
Zhao, CJ, Li, N and Deng, XM (2003) The establishment of method for identifying SNP genotype by CRS-PCR. Hereditas 25: 327–329.Google ScholarPubMed
Zhao, GF, Chen, H, Lei, CZ, et al. (2007) Study on SNPs of GHR gene and its associations with growth traits in Qinchuan cattle. Hereditas 29: 319–323.Google Scholar