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Creation of trivalent transgenic watermelon resistant to virus infection

Published online by Cambridge University Press:  12 February 2007

Niu Sheng-Niao ,
Huang Xue-Sen ,
Wong Sek-Man ,
Yu Jia-Lin ,
Zhao Fu-Xing ,
Li Da-Wei ,
Wang Sheng-You ,
Zhai Guang-Ming  and
Shi Fan-Sheng
Niu Sheng-Niao
Affiliation:
State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing, 100094, China
Huang Xue-Sen
Affiliation:
Shanxi Jinding Biology Seed Co. Ltd, Yuncheng, 044000, China
Wong Sek-Man
Affiliation:
National University of Singapore, Singapore, 117543
Yu Jia-Lin*
Affiliation:
State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing, 100094, China
Zhao Fu-Xing
Affiliation:
Shanxi Jinding Biology Seed Co. Ltd, Yuncheng, 044000, China
Li Da-Wei
Affiliation:
State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing, 100094, China
Wang Sheng-You
Affiliation:
Shanxi Jinding Biology Seed Co. Ltd, Yuncheng, 044000, China
Zhai Guang-Ming
Affiliation:
Shanxi Jinding Biology Seed Co. Ltd, Yuncheng, 044000, China
Shi Fan-Sheng
Affiliation:
Shanxi Jinding Biology Seed Co. Ltd, Yuncheng, 044000, China
*
*Corresponding author: Email: [email protected]
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Abstract

A trivalent vector containing genes of Watermelon mosaic virus (WMV) coat protein (CP), and replicase genes of Zucchini yellow mosaic virus (ZYMV) and Cucumber mosaic virus (CMV), was constructed for transformation of watermelon (Citrullus lanatus) plants, mediated by Agrobacterium tumefaciens. The integrated foreign genes were identified in the regenerated progenies by polymerase chain reaction (PCR) and Southern blots. The transformation efficiency was about 1.7‰. Resistance to virus infection was determined by mechanical inoculation in the greenhouse and by field trials. The transgenic watermelon lines showed different phenotypes of susceptible, resistant, immune or recovery from virus infections in the late growth stage. A relatively high level of resistance was shown by T3 plants of the line BH1-7. This result indicates the possibility of creating, by transgenic protocols, new varieties of watermelon resistant to viral infection.

Keywords

watermelonviral diseasestransformationresistance
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 3 , December 2005 , pp. 179 - 185
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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References

Dong, JZ and Jia, SR (1991) High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris Schard). Plant Cell Reports 9: 559–562.Google Scholar
Fuchs, M, McFerson, JR, Tricoli, DM et al. , (1997) Cantaloupe Line CZW-30 containing coat protein genes of Cucumber mosaic virus, Zucchini yellow mosaic virus and Watermelon mosaic virus -2 is resistant to those three viruses in the field. Molecular Breeding 3: 279–290.Google Scholar
Fuchs, M, Tricoli, DM, Carney, KJ et al. , (1998) Comparative virus resistance and fruit yield of transgenic squash with single and multiple coat protein genes. Plant Disease 80: 1350–1356.Google Scholar
Huang, XS, Jiao, DL and Na, L (1994) Watermelon plants regeneration by cotyledon in vitro culture. China Watermelon and Muskmelon 3: 15–16 (in Chinese).Google Scholar
Li, DW, Niu, SN, Han, CG et al. , (2000) Nucleotide sequence analysis of coat protein gene of Watermelon mosaic virus -2 Shanxi isolate. Journal of Henan Agricultural University 34: 337–340 (in Chinese with English abstract).Google Scholar
Quemada, H, Sieu, LC, Siemieniak, DR et al. , (1990) Watermelon mosaic virus II and Zucchini yellow mosaic virus: Cloning of 3'-terminal regions, nucleotide sequences, and phylogenetic comparisons. Journal of General Virology 71: 1451–1460.Google Scholar
Wan, L, Liu, JJ, Peng, XX et al. , (1996) Cloning and expression of coat protein gene of Watermelon mosaic virus 2 in Escherichia coli. Journal of Fruit Science 13: 5–10 (in Chinese with English abstract).Google Scholar
Wang, GL and Fang, HJ (1998) Principle and Technology of Plant Genetic Engineering. Beijing: Science Press (in Chinese).Google Scholar
Wang, HZ, Zhao, PJ, Xu, JC et al. , (2003) Virus resistance in transgenic watermelon plants containing a WMV-2 coat protein gene. Acta Genetica Sinica 30: 70–75 (in Chinese with English abstract).Google Scholar
Wang, HZ, Zhou, XY and Zhang, XN (1997) Studies on the transformation of Citrullus vulgaris Schrad and regeneration of transgenic plants. Acta Biologicae Experimentalis Sinica 30: 453–456 (in Chinese with English abstract).Google Scholar
Wu, M, Yeong, CY, Lee, SC et al. , (1993) Nucleotide sequence of the 3′ half of Zucchini yellow mosaic virus (Singapore isolate) genome encoding the 4K protein, protease, polymerase and coat protein. Nucleic Acids Research 21: 1317.CrossRefGoogle ScholarPubMed
Xie, XM, Yu, JL, Zhang, L and Liu, Y (1997) Tobacco transformation and the resistance mediated by the defective replicase gene from Cucumber mosaic virus. In: Liu, Yi (editor) Studies on Plant Viruses and Its Control. Beijing: China Agricultural Scitech Press, pp. 298–304 (in Chinese).Google Scholar