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Construction of plant expression vector and analysis of herbicide resistance and salt tolerance of transgenic tobacco

Published online by Cambridge University Press:  12 February 2007

Wang Xian-Yan ,
Shan Xiao-Yi ,
Yang Ai-Fang  and
Zhang Ju-Ren
Wang Xian-Yan
Affiliation:
School of Life Science, Shandong University, Jinan 250100, China
Shan Xiao-Yi
Affiliation:
School of Life Science, Shandong University, Jinan 250100, China
Yang Ai-Fang
Affiliation:
School of Life Science, Shandong University, Jinan 250100, China
Zhang Ju-Ren*
Affiliation:
School of Life Science, Shandong University, Jinan 250100, China
*
*Corresponding author: Email: [email protected]
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Abstract

The plant expression vector pCAMBIA1300-AtNHX1-als was constructed by inserting the herbicide resistance gene als of Arabidopsis thaliana into the plasmid pCAMBIA1300-AtNHX1, which contains the AtNHX1 gene encoding the Na+/H+ antiport from the vacuolar membrane of A. thaliana. Transgenic tobacco plants were obtained via Agrobacterium-mediated transformation. PCR and Southern blot assay indicated that genes als and AtNHX1 had been integrated into the genome of the transgenic plants. The herbicide resistance and salt tolerance of transgenic plants increased by about 1000-fold and by 1.5% NaCl concentration, respectively, compared with controls. Herbicide resistance of the T1 progeny was evaluated by spraying transgenic plants with different concentrations of Luhuanglong at the four-leaf stage. Controls gradually died under 100 mg/l Luhuanglong whereas 73% of the T1 plants still survived at 500 mg/l Luhuanglong. Thus the plant expression vector pCAMBIA1300-AtNHX1-als could be used to improve the herbicide resistance and salt tolerance of crops.

Keywords

alsAtNHX1salt toleranceherbicide resistancetobacco
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 2 , August 2004 , pp. 85 - 91
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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References

Apse, MP, Aharon, GS and Snedden, WA (1999) Salt tolerance conferred by over expression of a vacuolar Na +/H+ antiport in Arabidopsis. Science 285: 1256–1258.CrossRefGoogle Scholar
Ausubel, FM, Brent, R and Kingston, RE (1999) Short Protocols in Molecular Biology. Beijing: Science Press, pp. 36–38.Google Scholar
Blumwald, E, Aharaon, GS and Apse, MP (2000) Sodium transport in plant cells. Biochimica et Biophysica Acta 1465: 140–151.CrossRefGoogle ScholarPubMed
Chaleff, RS and Mauvais, CJ (1984) Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science 224: 1443–1445.CrossRefGoogle ScholarPubMed
Garciadeblas, B, Rubio, F and Quintero, FJ et al. . (1999) The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast. Proceedings of the National Academy of Sciences of the USA 96: 1480–1485.Google Scholar
Harms, CT, Armour, SL and DiMaio, J et al. . (1992) Herbicide resistance due to amplification of a mutant acetohydroxyacid synthase gene. Molecular & General Genetics 233: 427–435.CrossRefGoogle ScholarPubMed
Haughn, GW and Somerville, CR (1986) Sulfonylurea-resistant mutants of Arabidopsis thaliana. Molecular & General Genetics 204: 430–434.CrossRefGoogle Scholar
Haughn, GW, Muzar, BJ and Smith, J et al. . (1988) Transformation with a mutant Arabidopsis acetolactate synthase gene renders tobacco resistant to sulfonylurea herbicides. Molecular & General Genetics 211: 266–271.CrossRefGoogle Scholar
Krulwich, TA (1983) Na + /H + antiporters. Biochimica et Biophysica Acta 726: 245–264.CrossRefGoogle ScholarPubMed
LaRossa, RA, Falco, SC, Mazur, BJ et al. . (1987) Microbiological identification and characterization of an amino acid biosynthetic enzyme as the site of sulfonylurea herbicide action. In: Le Baron, HM, Mumma, RO, Honeycutt, RC and, Duesing,, JH (editors) Biotechnology in Agricultural Chemistry. ACS Symposium Series No. 334. Washington, DC: American Chemical Society, pp. 190–203.CrossRefGoogle Scholar
Lu, SD (1999) Protocols of Modern Molecular Biology, 2nd ed. Beijing: Press of Peking Union Medical College, p. 623.Google Scholar
Luttge, U and Ratajczak, R (1997) The physiology, biochemistry and molecular biology of the plant vaculor ATPase. Advances in Botanical Research 25: 253–296.CrossRefGoogle Scholar
Mazur, BJ, Falco, SC (1989) The development of herbicide-resistant crops. Annual Review of Plant Physiology 40: 441–470.CrossRefGoogle Scholar
Mazur, BJ, Chui, CF, Smith, JK (1987) Isolation and characterization of plant genes coding for acetolactate synthase, the target enzyme for two classes of herbicide. Plant Physiology 85: 1110–1117.CrossRefGoogle Scholar
Odell, JT, Caimi, PG, Yadav, NS et al. . (1990) Comparison of increased expression of wild-type and herbicide-resistant acetolactate synthase genes in transgenic plants, and indication of posttranscriptional limitation on enzyme activity. Plant Physiology 94: 1647–1654.CrossRefGoogle ScholarPubMed
Ren ZH, Ma, XL Zhao, YX et al. . (2002) Na+ /H + antiporter and plant salt tolerance. Chinese Journal of Biotechnology 18: 16–19.Google ScholarPubMed
Sun, YR (1989) Genetics Handbook Changsha: Science and Technology Press of Hunan, 450–452.Google Scholar
Yang, AF, Zhao, SL, Zhu, LP et al. . (2002) The creation of new sugar beet germplasm tolerant to salt by genetic transformation. Shandong Agricultural Science 2: 3–6.Google Scholar