Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-25T13:28:25.087Z Has data issue: false hasContentIssue false
  • English
  • Français

Cloning of a cDNA fragment of an antenna-specific gene in Helicoverpa armigera

Published online by Cambridge University Press:  12 February 2007

Wang Gui-Rong ,
Guo Yu-Yuan  and
Wu Kong-Ming
Wang Gui-Rong
Affiliation:
State Key Laboratory of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Guo Yu-Yuan
Affiliation:
State Key Laboratory of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Wu Kong-Ming*
Affiliation:
State Key Laboratory of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The olfactory sensors of insects' antennae, which are directly exposed to the environment, play an important role in identifying volatile hydrophobic molecules in the air. As many odours in the air are cytotoxic xenobiotics, there must be mechanisms in insects' antennae to decompose the molecules and also redundant odorant stimulators. In the present study, differential display PCR was used to isolate three antenna-specific mRNAs from the moth of the cotton bollworm, Helicoverpa armigera. Sequence analysis of the A4-1 clone indicated that it was homologous to the glutathione-S-transferase (GST) protein family. Northern blotting showed that the A4-1 clone was expressed specifically in the antennae of H. armigera and the expressed level was higher in male antennae than in female ones; this is similar to the expression of PBP-Harm gene from cotton bollworm. According to the gene's expression in antennae and the role of GST reported in other insects, the gene is predicted to be responsible for the decomposition of pheromones and harmful xenobiotics in antennae.

Keywords

Helicoverpa armigeradifferential display PCRglutathione-S-transferaseolfactionsex pheromone
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 1 , April 2004 , pp. 37 - 43
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Gershenzon, J and Croteau, R (1991) Terpenoids. In: Rosenthal, GA and Berenbaum, MR (editors) Herbivores: Their Interactions with Secondary Plant Metabolites. San Diego, CA: Academic Press, 165–209.CrossRefGoogle Scholar
Guo, YY (1998) Research of Helicoverpa armigera. Beijing: China Agricultural Sciences Press, 82–112.Google Scholar
Hayes, JD and Pulford, DJ (1995) The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Critical Reviews in Biochemistry and Molecular Biology 30: 445–600.CrossRefGoogle ScholarPubMed
Hovemann, BT, Sehlmeyer, F and Malz, J (1997) Drosophila melanogaster NADPH-cytochrome P450 oxidoreductase: pronounced expression in antennae may be related to odorant clearance. Gene 189: 213–219.CrossRefGoogle ScholarPubMed
Karlin, S and Altschul, SF (1990) Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes. Proceedings of the National Academy of Sciences of the USA 87: 2264–2268.CrossRefGoogle ScholarPubMed
Li, F and Prestwich, GD (1997) Expression and characterization of a lepidopteran general odorant binding protein. Insect Biochemistry and Molecular Biology 27: 405–412.Google Scholar
Mu, LY (1991) Research Method of Phytochemistry Protection. Beijing: China Agricultural Sciences Press 160–161.Google Scholar
Pelosi, P (1996) Perireceptor events in olfaction. Journal of Neurobiology 30: 3–19.3.0.CO;2-A>CrossRefGoogle ScholarPubMed
Pickett, CB and Lu, AYH (1989) Glutathione S-transferase: gene structure, regulation and biological function. Annual Review of Biochemistry 58: 743–764.CrossRefGoogle ScholarPubMed
Rogers, ME, Jani, MK and Vogt, RG (1999) An olfactory-specific glutathione-S-transferase in the sphinx moth, Manduca sexta. Journal of Experimental Biology 202: 1625–1637.CrossRefGoogle ScholarPubMed
Rybczynski, R, Reagan, J and Lerner, MR (1989) A pheromone-degrading aldehyde oxidase in the antennae of the moth Manduca sexta. Journal of Neuroscience 9: 1341–1353.CrossRefGoogle ScholarPubMed
Rybczynski, R, Vogt, RG and Lerner, MR (1990) Antennal-specific pheromone-degrading aldehyde oxidases from the moths Antheraea polyphemu s and Bombyx mori. Journal of Biological Chemistry 265: 19712–19715.CrossRefGoogle ScholarPubMed
Sambrook, KJ, Fritsch, EF and Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory Press.Google Scholar
Snyder, MJ and Maddison, DR (1997) Molecular phylogeny of glutathione-S-transferases. DNA and Cell Biology 16: 1373–1384.CrossRefGoogle ScholarPubMed
Synder, MJ, Walding, JK and Feyereisen, R (1995) Glutathione S-transferases from larval Manduca sexta midgut: sequence of two cDNAs and enzyme induction. Insect Biochemistry and Molecular Biology 25: 455–465.CrossRefGoogle Scholar
Tang, Z-H and Wu, S-X (1999) Inheritance and Evolution of Pesticidal Resistance in Insects. Shanghai: Shanghai Science and Technique Literature Press, pp. 157–190.Google Scholar
Tingle, FC and Mitchell, ER (1992) Attraction of Heliothis virescens to volatiles from extracts of cotton flowers. Journal of Chemical Ecology 18: 907–914.CrossRefGoogle ScholarPubMed
Visser, JH (1986) Host odor perception in phytophagous insects. Annual Review of Entomology 31: 121–144.CrossRefGoogle Scholar
Vogt, RG and Riddiford, LM (1981) Pheromone binding and inactivation by moth antennae. Nature 293: 161–163.CrossRefGoogle ScholarPubMed
Vogt, RG, Riddiford, LM and Prestwich, GD (1985) Kinetic properties of a sex pheromone-degrading enzyme: the sensillar esterase of Antheraea polyphemus. Proceedings of the National Academy of Sciences of the USA 82: 8827–8831.CrossRefGoogle ScholarPubMed
Wang, GR, Guo, YY and Wu, KM (2001) Partial cloning and characterization of the cDNA of general odorant-binding protein 1 gene in the antenna of Helicoverpa armigera (Hübner). Acta Entomologica Sinica 8: 289–297. (in Chinese with English abstract)Google Scholar
Wang, GR, Guo, YY, Xu, G and Wu, K (2002) Cloning and sequencing of a gene encoding GOBP2 in the antenna of Spodoptera exigua. Agricultural Sciences in China 1: 200–206. (in Chinese with English abstract)Google Scholar