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Analysis of isozymes related to energy metabolism of adult Tegillarca granosa

Published online by Cambridge University Press:  02 August 2007

Su Xiu-Rong ,
Lv Zhen-Ming ,
Li Tai-Wu ,
Liu Zhi-Ming  and
Paul K. Chien
Su Xiu-Rong*
Affiliation:
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China
Lv Zhen-Ming
Affiliation:
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China
Li Tai-Wu
Affiliation:
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China
Liu Zhi-Ming
Affiliation:
Department of Biology, Eastern New Mexico University, NM 88130, USA
Paul K. Chien
Affiliation:
Department of Biology, San Francisco University, CA 23430, USA
*
*Corresponding author. E-mail: [email protected]
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Abstract

The isozymes of 10 enzymes connected with energy metabolism in Tegillarca granosa were analysed by vertical polyacrylamide gel electrophoresis. Esterase and α-amylase are enzymes related to energy intake, their activities were high in the digestive gland. Malate dehydrogenase, malic enzyme, isocitrate dehydrogenase, succinate dehydrogenase, alcohol dehydrogenase, lactate dehydrogenase, 6-phosphogluconate dehydrogenase (G-6-PDH) and adenosine triphosphatase (ATPase) are enzymes related to energy metabolism. The main energy supply of T. granosa comes from aerobic respiration; anaerobic metabolism and the pentose phosphate pathway take an auxiliary role in energy metabolism. The high activity of G-6-PDH in T. granosa might mean a considerable proportion of carbohydrates metabolized through this pathway. This reaction could provide abundant NADP for metabolism in T. granosa. Compared with other shellfish, T. granosa had lower activity of ATPase, which might have some relationship with the amnicolous life and low motility of this animal.

Keywords

Tegillarca granosaenergy metabolismisozyme
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 2 , August 2007 , pp. 163 - 166
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(2): 242–246

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