Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-23T12:01:59.140Z Has data issue: false hasContentIssue false

Analysis of isozymes related to energy metabolism of adult Tegillarca granosa

Published online by Cambridge University Press:  02 August 2007

Su Xiu-Rong*
Affiliation:
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China
Lv Zhen-Ming
Affiliation:
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China
Li Tai-Wu
Affiliation:
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China
Liu Zhi-Ming
Affiliation:
Department of Biology, Eastern New Mexico University, NM 88130, USA
Paul K. Chien
Affiliation:
Department of Biology, San Francisco University, CA 23430, USA
*
*Corresponding author. E-mail: [email protected]

Abstract

The isozymes of 10 enzymes connected with energy metabolism in Tegillarca granosa were analysed by vertical polyacrylamide gel electrophoresis. Esterase and α-amylase are enzymes related to energy intake, their activities were high in the digestive gland. Malate dehydrogenase, malic enzyme, isocitrate dehydrogenase, succinate dehydrogenase, alcohol dehydrogenase, lactate dehydrogenase, 6-phosphogluconate dehydrogenase (G-6-PDH) and adenosine triphosphatase (ATPase) are enzymes related to energy metabolism. The main energy supply of T. granosa comes from aerobic respiration; anaerobic metabolism and the pentose phosphate pathway take an auxiliary role in energy metabolism. The high activity of G-6-PDH in T. granosa might mean a considerable proportion of carbohydrates metabolized through this pathway. This reaction could provide abundant NADP for metabolism in T. granosa. Compared with other shellfish, T. granosa had lower activity of ATPase, which might have some relationship with the amnicolous life and low motility of this animal.

Type
Research Article
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(2): 242–246

References

Choh, MS, Yap, CK, Tan, SG and Jambari, HA (2006) Morphological and allozyme studies of small terrestrial snails (Opeas sp., Subulina sp. and Huttonella bicolor) collected from Peninsular Malaysia. Genetika 42(1): 4957.Google ScholarPubMed
Hu, NS and Wan, XG (1985) The Technology of Isozymes and its Application. Hunan: Science and Technology Press, pp. 7085.Google Scholar
Li, TW, Sun, XQ and Liu, Y (2002) Biochemical genetic analysis of six isozymes on Chlamys farreri. Oceanologia et Limnologia Sinica 33(3): 232238.Google Scholar
Li, TW, Li, CH, Song, LS and Su, XR (2003) RAPD variation within and among five populations of Tegillarca granosa. Biodiversity Science 11(2): 118124.Google Scholar
Oliveira, ME, Russo, CA, Lazoski, C, Vianna, PR and Sole-Cava, AM (2005). Genetic variation and population structure of two species of neo-tropical mud-mussels (Mytella spp.). Genetic Molecular Research 30(2): 197202.Google Scholar
Robainas, BA, Espinosa, LG and Hernandez, D (2005) Temporal variation of the population structure and genetic diversity of Farfantepenaeus notialis assessed by allozyme loci. Molecular Ecology 14(10): 29332942.Google Scholar
Sun, JL, Zang, YP and Gao, H (2002) The effect of pH and temperature on protease activity in Tegillarca granosa. Fisher Science 21(6): 79.Google Scholar
van Riel, P, Jordaens, K, van Houtte, N and Martins, AM (2005) Molecular systematics of the endemic Leptaxini (Gastropoda: Pulmonata) on the Azores islands. Molecular. Phylogenetic Ecology 37(1): 132143.CrossRefGoogle ScholarPubMed
Wang, RC, Wang, ZP and Zhang, JZ (1993) Mariculture of Shellfish. Qing Dao: Ocean University of Qingdao Press, pp. 275290.Google Scholar
Wang, ZR (1996) Plant Allozyme Analysis. Beijing: Science Press, pp. 95112 (in Chinese).Google Scholar
Zasavskaya, NI and Pudovkin, AI (2005) Macrogeographic genetic variability in the gastropod mollusk Littorina sitkana from the northwest Pacific. Genetika 41(3): 374384.Google Scholar
Zhang, YP, Sun, JL and Zhou, HB (2003) Seasonal changes in activity of some digestive enzymes in different ages of Tegillarca granosa. Chinese Journal of Zoology 38(4): 2831.Google Scholar