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Analysis, gene cloning and expression of two α-amylases from Bacillus cereus

Published online by Cambridge University Press:  30 October 2009

Zhang Lin-lin ,
Wang Qi ,
Li Rong-xi ,
Wang Yong-jun  and
Mei Ru-hong
Zhang Lin-lin
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100094, China Department of Agronomy, Inner Mongolia Agricultural University, Huhehaote 010018, China
Wang Qi*
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100094, China
Li Rong-xi
Affiliation:
Department of Agronomy, Inner Mongolia Agricultural University, Huhehaote 010018, China
Wang Yong-jun
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100094, China
Mei Ru-hong
Affiliation:
Department of Agronomy, Inner Mongolia Agricultural University, Huhehaote 010018, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Bacillus cereus can produce α-amylase, which has important industrial production value and can endure high temperatures. The enzymatic characteristics of two α-amylases from B. cereus (B905 and B904) were studied. The results showed that both preserved their activity at 90–100°C. Their thermal stability and enzymatic activity did not depend on Ca2+. However, their protein molecular weights were obviously different. The structural genes of amy905 and amy904 were cloned successfully using the polymerase chain reaction (PCR) and expressed in Escherichia coli. The amy904 and amy905 genes were 1362 bp and 1761 bp long and their protein molecular weights were about 55 kDa and 68 kDa, respectively.

Keywords

Bacillus cereusα-amylasegene clonegene expression
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 2 , August 2009 , pp. 135 - 140
Copyright
Copyright © China Agricultural University 2009

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