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AFLP markers for genomic DNA fingerprinting in pigs
Published online by Cambridge University Press: 12 February 2007
Abstract
AFLP provides an effective, rapid and economical tool for detecting a large number of polymorphic genetic markers that are highly reliable and reproducible, and are able to be genotyped automatically. The AFLP technique has been used extensively to detect genetic polymorphisms, evaluate and characterize breed resources, construct genetic maps and identify genes. In this paper, we describe the optimization of the AFLP technique for porcine genomic DNA fingerprinting, including the enzyme digestion, adapter ligation, preamplification, selective amplification, denatured PAGE, silver staining and multicolour fluorescent detection. Twenty-eight polymorphic markers were detected in the pooled genomic DNA of 44 pig breeds (populations) by E32/T32 primer combinations.
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- Copyright © China Agricultural University and Cambridge University Press 2004