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Variation analysis of the VP1 and 3ABC genes from yellow cattle isolates persistently infected by Foot-and-mouth disease virus

Published online by Cambridge University Press:  29 January 2010

Shen Xiao-yan ,
Cong Guo-zheng ,
Chang Hui-yun ,
Liu Xiang-tao  and
Xie Qing-ge
Shen Xiao-yan
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National FMD Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Cong Guo-zheng
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National FMD Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Chang Hui-yun*
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National FMD Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Liu Xiang-tao
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National FMD Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Xie Qing-ge
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National FMD Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

The potential relationship between the establishment of Foot-and-mouth disease virus (FMDV) persistent infection and gene variation was identified by investigating the variation of VP1 and 3ABC genes from yellow cattle persistent infection isolates. Five yellow cattle were inoculated on their tongue with 1.0×104 ID50/ml of FMDV O/Akesu/58 strain. After displaying clinical or subclinical signs, they probably became asymptomatic carriers. Oesophageal–pharyngeal fluids were collected monthly from the carriers with a probang and inoculated into a baby hamster kidney cell line (BHK-21); 12 FMDV isolates were obtained. The VP1 and 3ABC genes of the 12 isolates were then amplified by reverse-transcriptase polymerase chain reaction (RT-PCR). Cloning and sequencing revealed that the homology of the VP1 nucleotide and amino-acid sequence of all the isolates was above 98%, with no base deletion or insertion. When compared with the O/Akesu/58 FMDV strain, the homology of the VP1 nucleotide sequence of the isolates was only 85%, and that of the deduced amino-acid sequence only 90%.There were several nucleotide mutations in the VP1 gene of the isolates, including 16 consistent nucleotide mutations, with only two of them leading to a change in amino acid (I56→T, A210→E). Moreover, it was found that four nucleotide points and three amino-acid points had transversions among all isolates. The 3ABC gene had only 13 nucleotide transversions and five amino-acid mutations. It was presumed that persistent FMDV infection might have little connection with variation in the VP1 and 3ABC genes, and was probably related to other structural protein gene and key factors.

Keywords

Foot-and-mouth disease virusyellow cattlepersistent infectiongene variation
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 3 , December 2009 , pp. 191 - 197
Copyright
Copyright © China Agricultural University 2009

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References

Alexandersen, S, Zhang, Z and Donaldson, AI (2002) Aspects of the persistence of Foot-and-mouth disease virus in animals: the carrier problem. Microbes and Infection 4: 1099–1110.CrossRefGoogle ScholarPubMed
Beard, CW and Mason, PW (2000) Genetic determinants of altered virulence of Taiwanese Foot-and-mouth disease virus. Journal of Virology 74: 987–991.CrossRefGoogle ScholarPubMed
Belsham, GJ, McInery, GM and Ross-Smith, N (2000) Foot-and-mouth disease virus 3C protease induces cleavage of translation initiation factors eIF4A and eIF4G within infected cells. Journal of Virology 74: 272–280.CrossRefGoogle ScholarPubMed
Dĺez, J, Hofner, M and Domingo, E (1990) Foot-and-mouth disease virus strains isolated from persistently infected cell cultures are attenuated for mice and cattle. Virus Research 18: 3–7.Google Scholar
Escarmis, C, Toja, M, Medina, M and Domingo, E (1992) Modifications of the 5′ untranslated region of Foot-and-mouth disease virus after prolonged persistence in cell culture. Virus Research 26: 113–125.CrossRefGoogle ScholarPubMed
Falk, MM, Grigera, PR and Bergmann, IE (1990) Foot-and-mouth disease virus protease 3C induces specific proteolytic cleavage of host cell histone H3. Journal of Virology 64: 748–756.CrossRefGoogle ScholarPubMed
Gebauer, F, De la Torre, JC and Gomes, I (1988) Rapid selection of genetic and antigenic variants of foot-and-mouth disease virus during persistence in cattle. Journal of Virology 62: 2041–2049.CrossRefGoogle ScholarPubMed
Geysem, HM, Meloem, RM and Barteling, SJ (1984) Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proceedings of the National Academy of Sciences of the USA 81: 3998–4002.CrossRefGoogle Scholar
Haydon, DT, Samuel, AR and Knowles, NJ (2001) The generation and persistence of genetic variation in Foot-and-mouth disease virus. Preventive Veterinary Medicine 51: 111–124.CrossRefGoogle ScholarPubMed
Horsington, J and Zhang, ZD (2007) Consistent change in the B–C loop of VP2 observed in Foot-and-mouth disease virus from persistently infected cattle: implication for association with persistence. Virus Research 125: 114–118.CrossRefGoogle ScholarPubMed
Huang, CC, Lee, F, Tu, WJ, et al. (2003) Anti3AB antibodies in the Chinese yellow cattle infected by the O/Taiwan/99 foot and mouth disease virus. Veterinary Microbiology 84: 317–326.CrossRefGoogle Scholar
Knowles, NJ, Davies, PR, Henry, T, O'Donnell, V, Pacheco, JM and Mason, PW (2001) Emergence in Asia of Foot-and-mouth disease virus with altered host range: characterization of alterations in the 3A protein. Journal of Virology 75: 1551–1556.CrossRefGoogle ScholarPubMed
Martín Hernández, AM, Carrillo, EC and Sevilla, N (1994) Rapid cell variation can determine the establishment of a persistent viral infection. Proceedings of the National Academy of Science USA 91: 3705–3709.CrossRefGoogle ScholarPubMed
Strohmaier, K, Fraze, R and Adam, KH (1982) Location and characterization of antigenic protein of the FMDV immunizing protein. The Journal of General Virology 59: 295–306.CrossRefGoogle ScholarPubMed
Toja, M, Escarmis, C and Domingo, E (1999) Genomic nucleotide sequence of a Foot-and-mouth disease virus clone and its persistent derivatives. Implications for the evolution of viral quasispecies during a persistent infection. Virus Research 64: 161–171.CrossRefGoogle ScholarPubMed
Weddel, GN, Yansura, DG and Dowbenko, DJ (1985) Sequence variation in the gene for the immunogenic capsid protein VP1 of Foot-and-mouth disease virus type A. Proceedings of the National Academy of Sciences of the USA 82: 2618–2622.CrossRefGoogle Scholar
Zhang, XS (2002) Sequencing for the Full-Length Genome of Foot-and-mouth disease virus China/99 and the Analysis of its Structure and Function (D). Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (in Chinese).Google Scholar
Zhang, ZD and Kitching, RP (2001) The localization of persistent Foot-and-mouth disease virus in the epithelial cells of the soft palate and pharynx. Journal of Comparative Pathology 124: 89–94.CrossRefGoogle ScholarPubMed
Zhang, YG, Lv, JL and Wang, YL (2004) Studies on variations of 3A and VP1 genes of Foot-and-mouth disease virus strain O/China/99 from serial passages in different hosts. Chinese Journal of Virology 20: 338–346 (in Chinese with English abstract).Google Scholar