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Purification and antibacterial activity of recombinant human lactoferrin in milk of transgenic mice

Published online by Cambridge University Press:  29 January 2010

Miao Ming-Xing ,
Yuan Yu-Guo ,
An Li-You ,
Zhao Jun-Hui ,
Bai Ya-Jun ,
Guo Lei  and
Cheng Yong
Miao Ming-Xing
Affiliation:
School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
Yuan Yu-Guo
Affiliation:
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
An Li-You
Affiliation:
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
Zhao Jun-Hui
Affiliation:
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
Bai Ya-Jun
Affiliation:
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
Guo Lei
Affiliation:
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
Cheng Yong*
Affiliation:
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
*
*Corresponding author: E-mail: [email protected]
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Abstract

To verify its antibacterial activity, recombinant human lactoferrin (rhLF) was extracted from the milk of transgenic mice (Mus musculus) (PCL25 and AP) by gel filtration chromatography and analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and enzyme-linked immunosorbent assay (ELISA). In addition, its bacteriostatic properties were tested using the agar disc diffusion method. ELISA analysis showed that the concentration of rhLF in the milk of transgenic mice ranged from 7 to 8 mg/ml, and the recombinant protein expressed in the milk had the same molecular weight as the native protein (~78 kDa), indicating that the rhLFs had a strong antibacterial activity on Escherichia coli and Salmonella.

Keywords

human lactoferrintransgenic micebacteriostatic properties
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 3 , December 2009 , pp. 253 - 256
Copyright
Copyright © China Agricultural University 2009

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