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Polymorphism of MC4R Asp298Asn site and its relationship with backfat thickness in commercial pigs

Published online by Cambridge University Press:  03 March 2009

Yang Xiao-Hui ,
Liu Yuan ,
Tang Hui ,
Zhang Ning-Bo ,
Wu Ying ,
Wei Shu-Dong  and
Jiang Yun-Liang
Yang Xiao-Hui
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Liu Yuan
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Tang Hui
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Zhang Ning-Bo
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
Wu Ying
Affiliation:
Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China
Wei Shu-Dong
Affiliation:
Bureau of Livestock of Laiwu City, Shandong Province, Laiwu 271100, China
Jiang Yun-Liang*
Affiliation:
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Melanocortin-4 receptor (MC4R) is a member of the superfamily of G-protein-coupled receptors (GPCRs), which affects body weight, energy homeostasis and food intake in humans and mice. In this study, the Asp298Asn polymorphism of the MC4R gene was investigated in Laiwu, Yorkshire×Laiwu and commercial cross-bred pig populations using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP), and the relationship of this mutation with backfat thickness was analysed. The results indicated that only genotype 11 exists in 33 individuals of Laiwu pigs, and three genotypes (11, 12 and 22) were detected in Yorkshire×Laiwu and commercial cross-bred populations. The distributions of allele and genotype frequencies in Yorkshire×Laiwu and commercial cross-bred populations were similar, with the frequency of allele 1 being higher than that of allele 2. In commercial cross-bred pigs, the mean backfat thickness of individuals with genotype 22 was significantly higher than that of individuals with genotypes 12 (P<0.01) and 11 (P<0.05). This study provides evidence that the Asp298Asn polymorphism of the MC4R gene is associated with backfat thickness in commercial cross-bred pigs with Western pigs as parental lines and, therefore, can be used as a DNA marker for breeding in such populations of pigs.

Keywords

pigMC4R genePCR-RFLPbackfat thickness
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 3 , December 2008 , pp. 251 - 255
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(3): 407–411

References

Chen, M, Wang, AG, Fu, JL and Li, N (2004) Difference allele frequencies MC4R gene variants in Chinese pig breeds. Archives of Tierzucht Dummerstorf 47: 463–468.Google Scholar
Costa, JL, Brennen, MB and Hochgeschwender, U (2002) The human genetics of eating disorders: lessons from the leptin/melanocortin system. Child and Adolescent Psychiatric Clinics of North America 11(2): 387–397.CrossRefGoogle ScholarPubMed
Geller, F, Reichwald, K, Dempfle, A, et al. (2004) Melanocortin-4 receptor gene variant I103 is negatively associated with obesity. American Journal of Human Genetics 74(3): 572–581.CrossRefGoogle ScholarPubMed
Hernández-Sánchez, J, Visscher, P, Plastow, G and Haley, C (2003) Candidate gene analysis for quantitative traits using the transmission disequilibrium test: The example of the melanocortin four receptor in pigs. Genetics 164: 637–644.CrossRefGoogle Scholar
Hoggard, N, Hunter, L, Duncan, JS and Rayner, DV (2004) Regulation of adipose tissue leptin secretion by alpha-melanocyte-stimulating hormone and agouti-related protein: further evidence of an interaction between leptin and the melanocortin signalling system. Molecular Endocrinology 32(1): 145–153.CrossRefGoogle ScholarPubMed
Houston, RD, Cameron, ND and Rance, KA (2004) A melanocortin four receptor (MC4R) polymorphism is associated with performance traits in divergently selected large white pig populations. Animal Genetics 35(5): 386–390.CrossRefGoogle Scholar
Huszar, D, Lynch, CA and Fairchild-Huntress, V (1997) Targeted disruption of the melanocortin-4-receptor results in obesity in mice. Cell 88(1): 131–141.CrossRefGoogle ScholarPubMed
Jokubka, R, Maak, S, Kerziene, S and Swalve, HH (2006) Association of a melanocortin 4 receptor (MC4R) polymorphism with performance traits in Lithuanian White pigs. Journal of Animal Breeding and Genetics 123: 17–22.CrossRefGoogle ScholarPubMed
Kim, KS, Larsen, N and Rothschild, MF (2000a) Linkage and physical mapping of the porcine melanocortin-4 receptor (MC4R) gene. Journal of Animal Science 78: 791–792.CrossRefGoogle ScholarPubMed
Kim, KS, Larsen, N, Short, T, Plastow, G and Rothschild, MF (2000b) A missense variant of the porcine melanocortin-4 receptor (MC4R) gene is associated with fatness, growth, and feed intake traits. Mammalian Genome 11: 131–135.CrossRefGoogle ScholarPubMed
Li, CL, Pan, YC, Meng, H, Wang, ZL and Huang, XG (2006) Distributions of polymorphism of ADD1, MC4R and H-FABP genes associated with IMF and BF in three populations in pig. Hereditas(Beijing) 28(2): 159–164 (in Chinese with English abstract).Google ScholarPubMed
Liu, GL, Jiang, SW, Xiong, YZ, Zheng, R and Qu, YC (2002) Molecular screening of MC4R gene and association with fat traits in pig resource family. Acta Genetica Sinica 29(6): 497–501 (in Chinese with English abstract).Google ScholarPubMed
Ovilo, C, Fernandez, A, Rodríguez, MC, Nieto, M and Silio, L (2006) Association of MC4R gene variants with growth, fatness, carcass composition and meat and fat quality traits in heavy pigs. Meat Science 73: 42–47.CrossRefGoogle ScholarPubMed
Park, HB, Carlborg, O, Marklund, S and Andersson, L (2002) Melanocortin-4 receptor (MC4R) genotypes have no major effect on fatness in a Large White×Wild Boar intercross. Animal Genetics 33: 155–157.CrossRefGoogle Scholar
Sambrook, J, Fritsch, EF and Maniatis, T (1989) Molecular cloning: A Laboratory Manual, 2nd ed. New York: Cold Spring Harbor Laboratory Press.Google Scholar
Schwartz, MW and Woods, SC (2000) Central nervous system control of food intake. Nature 404(6778): 661–671.CrossRefGoogle ScholarPubMed
Sinha, PS, Schioth, HB and Tatro, JB (2004) Roles of the melanocortin-4 receptor in antipyretic and hyperthermic actions of centrally administered alpha-MSH. Brain Research 1001(1): 150–158.CrossRefGoogle ScholarPubMed
Stachowiak, M, Szydlowski, M, Obarzanek-Fojt, M and Switonski, M (2005) An effect of a missense mutation in the porcine melanocortin-4 receptor (MC4R) gene on production traits in Polish pig breeds is doubtful. Animal Genetics 37: 55–57.Google Scholar
Xiao, SJ, Yan, Y, Ren, J, et al. (2006) Polymorphisms of MC4R causative mutation in Chinese and western pig breeds and its effects on growth and fatness traits. Acta Veterinaria and Zootechnica Sinica 37(9): 841–845 (in Chinese with English abstract).Google Scholar
Yeo, GS, Farooqi, IS, Aminian, S, Halsall, DJ, Stanhope, RG and Rahilly, SO (1998) A frame shift mutation in MC4R associated with dominantly inherited human obesity. Nature Genetics 20(2): 111–112.CrossRefGoogle ScholarPubMed
Yeo, GS, Farooqi, IS, Challis, BG, Jackson, RS and Rahilly, SO (2000) The role of melanocortin signalling in the control of body weight: Evidence from human and murine genetic models. Quarterly Journal of Medicine 93(1): 7–14.CrossRefGoogle ScholarPubMed
Young, EH, Wareham, NJ, Farooqi, S, et al. (2007) The V103I polymorphism of the MC4R gene and obesity: Population based studies and meta-analysis of 29563 individuals. International Journal of Obesity 31(9): 1437–1441.CrossRefGoogle Scholar
Zhao, XF, Nie, GJ and Xu, NY (2004) Investigation on polymorphism of MC4R gene for Jinhua pig. Swine Production 5: 22–24 (in Chinese with English abstract).Google Scholar