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Microsatellite analysis of landrace rice core collection in Yunnan, China

Published online by Cambridge University Press:  12 February 2007

Zhang Hong-Liang ,
Li Zi-Chao ,
Liao Deng-Qun ,
Liu Xia ,
Zeng Ya-Wen ,
Shen Shi-Quan ,
Mu Ping ,
Yang Zhong-Yi  and
Wang Xiang-Kun
Zhang Hong-Liang
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
Li Zi-Chao*
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
Liao Deng-Qun
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
Liu Xia
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China College of Agronomics, Xinjiang Agricultural University, Urumchi 830052, China
Zeng Ya-Wen
Affiliation:
Crop Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
Shen Shi-Quan
Affiliation:
Crop Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
Mu Ping
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
Yang Zhong-Yi
Affiliation:
Crop Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
Wang Xiang-Kun
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

The distribution of genetic diversity between Oryza sativa L. ssp. indica and O. sativa L. ssp. japonica covering different ecological zones in Yunnan was studied, and specific markers of indica/japonica subspecies, paddy/upland rice and different ecological zones were screened, using 36 microsatellite primers and 113 accessions in the Yunnan landrace rice core collection. The genetic diversity of japonica was higher than that of indica, and the ecological zone with the highest and smallest genetic diversity lay in south-east and north-east Yunnan, respectively. This distribution was consistent at morphological and isozyme levels with studies on the entire Yunnan rice resources and core collection. In addition, the results showed that, among 416 markers, there were six indica/japonica-specific markers, 15 specific markers for paddy/upland rice and three specific markers in different ecological zones. The main conclusions are that the landrace rice core collection in Yunnan genetically represents the entire landrace rice resources in Yunnan, the centre of genetic diversity at DNA level lies in south-east Yunnan, and the DNA differentiation between indica and japonica is small. Furthermore, microsatellite markers were useful for studying the genetic diversity, classification and ecotype of germplasm resources and their core collection.

Keywords

core collectiongenetic diversitymicrosatellite markerrice germplasm resources
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 1 , April 2004 , pp. 23 - 30
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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