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Influence of maize Lc regulatory gene on flower colour of transgenic tobacco and petunia

Published online by Cambridge University Press:  02 August 2007

Yang Chi-Chun ,
Yu Jing-Juan ,
Zhao Qian ,
Zhu Deng-Yun  and
Ao Guang-Ming
Yang Chi-Chun
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
Yu Jing-Juan
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
Zhao Qian
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
Zhu Deng-Yun*
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
Ao Guang-Ming
Affiliation:
State Key Laboratory for Agrobiotechnology, College of Biology, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

The Lc regulatory gene affects the formation of anthocyanin in plants. XY355 promoter, a petal-specific promoter, was obtained from the genome of rape (Brassica napus) by polymerase chain reaction (PCR). A plant expression vector, pXY60, was constructed, which contained the maize Lc regulatory gene under the control of the XY355 promoter. The vector was introduced into tobacco (Nicotiana tabacum) and petunia (Petunia hybrida) by an Agrobacterium tumefaciens-mediated method. The flower colour of some transgenic tobacco plants was changed from light red to deep red and that of some transgenic petunia plants had changed from white to light purple.

Keywords

petal-specific promoterLc regulatory genetobaccopetuniaflower colour
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 2 , August 2007 , pp. 145 - 150
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 14(6): 85–89

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